Detection of hydroxy-nitro-PAHS (-polycyclic aromatic hydrocarbons) in an ambient air particulate extract using bioassay directed fractionation

Technical Report · Fri Nov 01 04:00:00 EST 1985

OSTI ID:6065368

Nishioka, M G; Howard, C C; Lewtas, J

Urban air particles contain extractable organic matter with both mutagenic and carcinogenic activity. Detection of the specific compounds responsible for mutagenic activity has been limited by the complexity of these extracts and the presence of highly polar components difficult to identify. To facilitate identifications, these extracts must be separated into substantially less-complex fractions and analyzed with newer analytical techniques. Bioassay-directed fractionation has been proposed as the most cost- and time-effective approach to identifying mutagenic compounds in complex mixtures by directing the analytical and identification research effort toward only significantly mutagenic fractions. This approach should be especially useful for the detection of heretofore unknown constituents of the matrix as the methods are not directed to or developed for specific compounds or compound classes.

• Research Organization: Battelle Columbus Labs., OH (USA)
• OSTI ID: 6065368
• Report Number(s): PB-86-130002/XAB
• Country of Publication: United States
• Language: English

Related Subjects

500200* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AIR POLLUTION
AROMATICS
BIOASSAY
BUILDINGS
CARCINOGENS
CHEMICAL ANALYSIS
DETECTION
DISTILLATION
EXTRACTION
HYDROCARBONS
INDUSTRIAL WASTES
MASS SPECTROSCOPY
MUTAGENS
ORGANIC COMPOUNDS
PARTICLES
PARTICULATES
POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS
RESIDENTIAL BUILDINGS
SEPARATION PROCESSES
SPECTROSCOPY
WASTES