Detection of hydroxylated nitro aromatic and hydroxylated nitro polycyclic aromatic compounds in an ambient air particulate extract using bioassay-directed fractionation

Journal Article · Mon Aug 01 04:00:00 EDT 1988 · Environ. Sci. Technol.; (United States)

DOI:https://doi.org/10.1021/es00173a007· OSTI ID:6861327

Nishioka, M G; Howard, C C; Contos, D A; Ball, L M; Lewtas, J

The identification and quantification of hydroxylated nitro aromatic compounds in an ambient air particulate extract are presented here. The isomer substitution observed for these compounds supports the mechanism proposed from smog chamber studies: OH attack on the aromatic compound followed by nitration ortho or para to the OH substitutent. Several hydroxylated nitro polycyclic aromatic compounds have been tentatively identified and quantified in the extract also. Both electron ionization (EI) and negative chemical ionization (NCI) high-resolution gas chromatography/mass spectrometry. (GC/MS) analyses were used to identify these components. Bioassay-directed fractionation through three sequential separations led to detection of the hydroxylated nitro polycyclic compounds in a mutagenic and substantially simplified subfraction of the extract.

• Research Organization: Battelle Columbus Division, Columbus, OH (USA)
• OSTI ID: 6861327
• Journal Information: Environ. Sci. Technol.; (United States), Journal Name: Environ. Sci. Technol.; (United States) Vol. 22:8; ISSN ESTHA
• Country of Publication: United States
• Language: English

Related Subjects

500200 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
AROMATICS
CHROMATOGRAPHY
FRACTIONATION
GAS CHROMATOGRAPHY
HYDROCARBONS
MASS SPECTROSCOPY
MUTAGEN SCREENING
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARTICLES
PARTICULATES
POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS
SCREENING
SEPARATION PROCESSES
SPECTROSCOPY