Detection of hydroxylated nitro aromatic and hydroxylated nitro polycyclic aromatic compounds in an ambient air-particulate extract using bioassay-directed fractionation

Technical Report · Fri Jan 01 04:00:00 EST 1988

OSTI ID:5165531

Nishioka, M G; Howard, C C; Contos, D A; Ball, L M; Lewtas, J

This report identifies hydroxylated nitro-polynuclear aromatic hydrocarbons (OH-NO{sub 2}PAHs) in an ambient air-particulate extract by electron impact (EI) and negative chemical ionization (NCI) high-resolution gas chromatography/mass spectrometry (HRGC/MS) analyses. Bioassay-directed fractionation through three sequential separations led to detection of these OH-NO{sub 2}-PAHs in a highly mutagenic and substantially simplified subfraction of the extract. The compound class identification was based on comparison of both EI and NCI mass spectra of reference OH-NO{sub 2}-pyrenes. The HRGC/MC retention values for seven OH-NO{sub 2}-fluoranthene isomer. Quantification of this compound class by NCI HRGC/MC with on-column injection is demonstrated with sensitivity to 0.5 ng/micro L.

• Research Organization: Battelle Columbus Labs., OH (USA)
• OSTI ID: 5165531
• Report Number(s): PB-89-237432/XAB
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201 -- Chemical & Physicochemical Properties
54 ENVIRONMENTAL SCIENCES
540120* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (1990-)
AIR POLLUTION
AMINES
AROMATICS
BIOASSAY
CHROMATOGRAPHY
DETECTION
DISTILLATION
GAS CHROMATOGRAPHY
HYDROCARBONS
MASS SPECTROSCOPY
ORGANIC COMPOUNDS
PARTICLES
PARTICULATES
POLLUTION
POLYCYCLIC AROMATIC AMINES
POLYCYCLIC AROMATIC HYDROCARBONS
SEPARATION PROCESSES
SPECTROSCOPY