Multiplexed fluorescence detector for capillary electrophoresis using axial optical fiber illumination

Journal Article · Thu Apr 01 04:00:00 EST 1993 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00055a021· OSTI ID:6062895

Taylor, J A; Yeung, E A ^[1]

---

1. Iowa State Univ., Ames (United States)

Recently, we developed an axial beam excitation scheme for capillary electrophoresis. We report the use of this excitation geometry to simultaneously monitor 10 capillary tubes undergoing electrophoresis. This represents a truly parallel multiplexing scheme for monitoring large arrays of capillaries. The system has no moving parts. Data rates are compatible with state-of-the-art run times in CGE and are fast enough even if there is another order of magnitude increase in DNA sequencing rates per lane in the future. 24 refs., 3 figs.

• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 6062895
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 65:7; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400100 -- Analytical & Separations Chemistry
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
CHROMOSOMES
DETECTION
DNA
DNA SEQUENCING
ELECTRONIC EQUIPMENT
ELECTROPHORESIS
ENERGY-LEVEL TRANSITIONS
EQUIPMENT
EXCITATION
FIBERS
FLUORESCENCE
LUMINESCENCE
MULTIPLEXERS
NUCLEIC ACIDS
OPTICAL FIBERS
ORGANIC COMPOUNDS
ORGANS
STRUCTURAL CHEMICAL ANALYSIS