Multiplexed fluorescence detector system for capillary electrophoresis

Title: Multiplexed fluorescence detector system for capillary electrophoresis

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Abstract

A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

Inventors:

Yeung, Edward S ^[1]; Taylor, John A ^[2]

Ames, IA
Nevada, IA

Publication Date:: 1996-03-12

Research Org.:: Ames Lab., Ames, IA (United States); Iowa State University, Ames, IA (US)

OSTI Identifier:: 870334

Patent Number(s):: US 5498324

Application Number:: 08/223,864

Assignee:: Iowa State University Research Foundation, Inc. (Ames, IA)

DOE Contract Number:: W-7405-ENG-82

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: multiplexed; fluorescence; detector; capillary; electrophoresis; detection; provided; simultaneously; excite; substantially; monitor; separations; multiple; capillaries; multiplexing; approach; involves; laser; irradiation; sample; plurality; optical; fibers; coupled; individually; array; imaged; orthogonally; microscope; charge-coupled; device; camera; signal; analysis; fluorescence detection; charge-coupled device; multiple capillaries; laser irradiation; coupled device; substantially simultaneously; capillary electrophoresis; optical fibers; optical fiber; signal analysis; approach involves; fluorescence detector; simultaneously monitor; simultaneously excite; multiplexed fluorescence; device camera; excite fluorescence; substantially simultaneous; /204/356/

Citation Formats


                    Yeung, Edward S, and Taylor, John A. Multiplexed fluorescence detector system for capillary electrophoresis.  United States: N. p., 1996. 
        Web.

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                    Yeung, Edward S, & Taylor, John A. Multiplexed fluorescence detector system for capillary electrophoresis.  United States.

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                    Yeung, Edward S, and Taylor, John A. Tue .  
        "Multiplexed fluorescence detector system for capillary electrophoresis".  United States.  https://www.osti.gov/servlets/purl/870334.

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@article{osti_870334,

  title        = {Multiplexed fluorescence detector system for capillary electrophoresis},

  author       = {Yeung, Edward S and Taylor, John A},

  abstractNote = {A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/870334},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1996},

  month        = {3}

}

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Multiplexed fluorescence detector system for capillary electrophoresis

Patent Yeung, Edward S ; Taylor, John A

A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.
Full Text Available
Multiplexed fluorescence detector system for capillary electrophoresis

Patent Yeung, E S ; Taylor, J A

A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figs.
Multiplexed fluorescence detector system for capillary electrophoresis

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A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figures.
Multiplexed fluorescence detector for capillary electrophoresis using axial optical fiber illumination

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Recently, we developed an axial beam excitation scheme for capillary electrophoresis. We report the use of this excitation geometry to simultaneously monitor 10 capillary tubes undergoing electrophoresis. This represents a truly parallel multiplexing scheme for monitoring large arrays of capillaries. The system has no moving parts. Data rates are compatible with state-of-the-art run times in CGE and are fast enough even if there is another order of magnitude increase in DNA sequencing rates per lane in the future. 24 refs., 3 figs.
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Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries

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Various excitation schemes for distributing a laser beam to a large number of capillaries in an array are evaluated. This led to the construction of a multiplexed system for monitoring the electrophoresis of DNA fragments in 100 capillaries. The laser-excited fluorescence signals from each capillary are simultaneously recorded at the rate of 0.6 frame/s by a CCD camera. The reconstructed electropherograms show excellent reproducibility and minimal cross-talk. The system provides for two simultaneous excitation wavelengths so that it can be adapted for two-color, two-intensity DNA sequencing based on the commercial four-dye chemistry. Only 20 mW per laser line was employed.more »« less
https://doi.org/10.1021/ac00081a010

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