Multiplexed fluorescence detector system for capillary electrophoresis

Yeung, Edward S; Taylor, John A

Multiplexed fluorescence detector system for capillary electrophoresis

Patent · Fri Dec 31 23:00:00 EST 1993

OSTI ID:869367

Yeung, Edward S ^[1]; Taylor, John A ^[2]

Ames, IA
Nevada, IA

A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

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Research Organization:: Ames Laboratory (AMES), Ames, IA; Iowa State University, Ames, IA (US)

DOE Contract Number:: W-7405-ENG-82

Assignee:: Iowa State University Research Foundation, Inc. (Ames, IA)

Patent Number(s):: US 5324401

Application Number:: 08/013,960

OSTI ID:: 869367

Country of Publication:: United States

Language:: English

References (16)

Gel-filled capillaries for nucleic acid separations in capillary electrophoresis Baba, Yoshinobu; Tsuhako, Mitsutomo TrAC Trends in Analytical Chemistry, Vol. 11, Issue 8 https://doi.org/10.1016/0165-9936(92)87090-7	journal	September 1992
Fluorescence detection in capillary zone electrophoresis using a charge-coupled device with time-delayed integration Sweedler, J. V.; Shear, J. B.; Fishman, H. A. Analytical Chemistry, Vol. 63, Issue 5 https://doi.org/10.1021/ac00005a022	journal	March 1991
Compensating for instrumental and sampling biases accompanying electrokinetic injection in capillary zone electrophoresis Lee, Thomas T.; Yeung, Edward S. Analytical Chemistry, Vol. 64, Issue 11 https://doi.org/10.1021/ac00035a007	journal	June 1992
Multiplex DNA sequencing Church, G.; Kieffer-Higgins, S. Science, Vol. 240, Issue 4849 https://doi.org/10.1126/science.3353714	journal	April 1988
Expert system for data acquisition to achieve a constant signal-to-noise ratio: application to imaging of DNA sequencing gels Koutny, Lance B.; Yeung, Edward S. Analytical Chemistry, Vol. 65, Issue 2 https://doi.org/10.1021/ac00050a010	journal	January 1993
Native fluorescence detection of nucleic acids and DNA restriction fragments in capillary electrophoresis Milofsky, Robert E.; Yeung, Edward S. Analytical Chemistry, Vol. 65, Issue 2 https://doi.org/10.1021/ac00050a011	journal	January 1993
Facilitating data transfer and improving precision in capillary zone electrophoresis with migration indexes Lee, Thomas T.; Yeung, Edward S. Analytical Chemistry, Vol. 63, Issue 24 https://doi.org/10.1021/ac00024a007	journal	December 1991
DNA sequencing using capillary array electrophoresis Huang, Xiaohua C.; Quesada, Mark A.; Mathies, Richard A. Analytical Chemistry, Vol. 64, Issue 18 https://doi.org/10.1021/ac00042a021	journal	September 1992
Capillary array electrophoresis using laser-excited confocal fluorescence detection Huang, Xiaohua C.; Quesada, Mark A.; Mathies, Richard A. Analytical Chemistry, Vol. 64, Issue 8 https://doi.org/10.1021/ac00032a025	journal	April 1992
Laser Fluorometric Detection in Liquid Chromatography Young, Edward S.; Sepaniak, Michael J. Analytical Chemistry, Vol. 52, Issue 13, p. 1465A-1481A https://doi.org/10.1021/ac50063a804	journal	November 1980
Three DNA sequencing methods using capillary gel electrophoresis and laser-induced fluorescence Swerdlow, Harold.; Zhang, Jian Zhong.; Chen, Da Yong. Analytical Chemistry, Vol. 63, Issue 24 https://doi.org/10.1021/ac00024a006	journal	December 1991
DNA sequencing, automation, and the human genome Trainor, George L. Analytical Chemistry, Vol. 62, Issue 5 https://doi.org/10.1021/ac00204a001	journal	March 1990
Temperature programming in capillary zone electrophoresis Whang, Chen Wen.; Yeung, Edward S. Analytical Chemistry, Vol. 64, Issue 5 https://doi.org/10.1021/ac00029a010	journal	March 1992
Axial-beam laser-excited fluorescence detection in capillary electrophoresis Taylor, John A.; Yeung, Edward S. Analytical Chemistry, Vol. 64, Issue 15 https://doi.org/10.1021/ac00039a020	journal	August 1992
High-performance charge transfer device detectors Sweedler, Jonathan V.; Bilhorn, Robert B.; Epperson, Patrick M. Analytical Chemistry, Vol. 60, Issue 4 https://doi.org/10.1021/ac00155a002	journal	February 1988
Applications of charge transfer devices in spectroscopy Epperson, Patrick M.; Sweedler, Jonathan V.; Bilhorn, Robert B. Analytical Chemistry, Vol. 60, Issue 5 https://doi.org/10.1021/ac00156a001	journal	March 1988

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Related Subjects

/204/356/
analysis
approach
approach involves
array
camera
capillaries
capillary
capillary electrophoresis
charge-coupled
charge-coupled device
coupled
coupled device
detection
detector
device
device camera
electrophoresis
excite
excite fluorescence
fibers
fluorescence
fluorescence detection
fluorescence detector
imaged
individually
involves
irradiation
laser
laser irradiation
microscope
monitor
multiple
multiple capillaries
multiplexed
multiplexed fluorescence
multiplexing
optical
optical fiber
optical fibers
orthogonally
plurality
provided
sample
separations
signal
signal analysis
simultaneously
simultaneously excite
simultaneously monitor
substantially
substantially simultaneous
substantially simultaneously

Multiplexed fluorescence detector system for capillary electrophoresis

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References (16)

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Related Subjects