The increased neutrophil adherence seen during reperfusion of ischemic skeletal muscle is not superoxide radical dependent
- S.I.U. School of Medicine, Springfield, IL (United States)
It has been hypothesized that the increase in neutrophil adherence seen in reperfused tissues following ischemia is a consequence of the generation of oxygen-derived free radicals. The authors have tested this hypothesis in an intravital microcirculatory preparation of rat gracilis muscle. The neutrophil adherence during reperfusion of ischemic muscle was quantitated following systemic administration of a superoxide free radical scavenger. Rats were assigned randomly to either a no treatment group or a group given a bolus of superoxide dismutase modified with polyethylene glycol (PEG-SOD) 10,000 u/Kg IV 15 minutes prior to reperfusion. On release of the clamp, and for 2 hours of reperfusion, the microcirculation was observed and recorded on video tape. The degree of neutrophil sticking (stationary for 30 seconds) in 100 micron segments of venules (diameters between 10 and 50 microns) was analyzed off-line. Results show that the average number of adherent cells within studied segments increased with time of reperfusion and this increase was greater for the 7 hour ischemic period as compared to the 5 hour ischemia. Muscle treated with PEG-SOD showed increases in the neutrophil adherence not significantly different from that seen in the no-treatment group. It was concluded that chemical messengers other than superoxide free radicals mediate the increase in neutrophil adherence seen during reperfusion of ischemic skeletal muscle.
- OSTI ID:
- 6058664
- Report Number(s):
- CONF-9104107--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 4:3; ISSN 0892-6638; ISSN FAJOE
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARDIOVASCULAR DISEASES
DISEASES
ENZYMES
HEMIC DISEASES
ISCHEMIA
LEUKOCYTES
MAMMALS
MATERIALS
MUSCLES
NEUTROPHILS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PATHOGENESIS
PROTEINS
RADICALS
RATS
RODENTS
SCAVENGING
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES