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Functional and structural evaluation of the vasculature of skin flaps after ischemia and reperfusion

Journal Article · · Plast. Reconstr. Surg.; (United States)
; ; ; ;

Free radicals and other toxic oxygen species play a role in the pathogenesis of ischemic organ damage. The abdominal skin flap has been used as a model to study the effects of superoxide dismutase on the survival of ischemic skin. We have evaluated the evolution of functional and structural injury to the vasculature after ischemic injury in superoxide dismutase-treated and control skin flaps. Ischemia was induced by creating abdominal skin flaps and occluding either the venous or both the venous and arterial blood supplies. Superoxide dismutase was administered immediately after the occlusion was released. At 1 hour of reflow, erythrocyte stasis, platelet deposition, neutrophil adherence, and injury to the endothelium of the large vessels and of the microvasculature were evident. The blood flow in the ischemic skin was only 3 percent of normal. Superoxide dismutase caused no change in the ultrastructure of the vasculature and a marginal decrease in vascular permeability in the ischemic skin at 1 hour of reflow. Increased fluorescent staining of the skin was evident after 24 hours of reflow in the superoxide dismutase-treated flaps. These findings indicate that injury to vascular endothelium by ischemia and reperfusion plays a role in the evolution of skin necrosis.

Research Organization:
Univ. of Maryland, Baltimore (USA)
OSTI ID:
7194858
Journal Information:
Plast. Reconstr. Surg.; (United States), Journal Name: Plast. Reconstr. Surg.; (United States) Vol. 81:5; ISSN PRSUA
Country of Publication:
United States
Language:
English

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Related Subjects

550901 -- Pathology-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABDOMEN
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BLOOD CELLS
BLOOD FLOW
BLOOD PLATELETS
BODY
BODY AREAS
BODY FLUIDS
CARDIOVASCULAR DISEASES
DISEASES
ENDOTHELIUM
ENZYMES
ISCHEMIA
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MORPHOLOGICAL CHANGES
NECROSIS
NEUTROPHILS
ORGANS
OXIDOREDUCTASES
PATHOGENESIS
PATHOLOGICAL CHANGES
PERMEABILITY
RADICALS
RADIOISOTOPES
RATS
RODENTS
SKIN
SUPEROXIDE DISMUTASE
TISSUES
TOXICITY
ULTRASTRUCTURAL CHANGES
VASCULAR DISEASES
VERTEBRATES