O2 free radicals: cause of ischemia-reperfusion injury to cardiac Na+-K+-ATPase

Journal Article · Sun Feb 01 04:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:6692919

Kim, M S; Akera, T

The role of O2 free radicals in the reduction of sarcolemmal Na+-K+-ATPase, which occurs during reperfusion of ischemic heart, was examined in isolated guinea pig heart using exogenous scavengers of O2 radicals and an inhibitor of xanthine oxidase. Ischemia and reperfusion reduced Na+-K+-ATPase activity and specific (3H)ouabain binding to the enzyme in ventricular muscle homogenates and also markedly lowered sodium pump activity estimated from ouabain-sensitive 86Rb+ uptake by ventricular muscle slices. These effects of ischemia and reperfusion were prevented to various degrees by O2-radical scavengers, such as superoxide dismutase, catalase, dimethyl-sulfoxide, histidine, or vitamin E or by the xanthine oxidase inhibitor, allopurinol. The degree of protection afforded by these agents paralleled that of reduction in enhanced lipid peroxidation of myocardial tissue as estimated from malondialdehyde production. These results strongly suggest that O2 radicals play a crucial role in the injury to sarcolemmal Na+-K+-ATPase during reperfusion of ischemic heart.

• Research Organization: Michigan State Univ., East Lansing
• OSTI ID: 6692919
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ALKALI METAL ISOTOPES
ANIMALS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CATALASE
DAYS LIVING RADIOISOTOPES
DISEASES
DMSO
DRUGS
ELEMENTS
ENZYMES
GLYCOSIDES
GUINEA PIGS
HEART
HYDROLASES
INFUSION
INTAKE
INTERMEDIATE MASS NUCLEI
ISCHEMIA
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MINUTES LIVING RADIOISOTOPES
MUSCLES
MYOCARDIUM
NONMETALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OUABAIN
OXIDOREDUCTASES
OXYGEN
PEROXIDASES
PHOSPHOHYDROLASES
RADICALS
RADIOISOTOPES
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
STROPHANTHINS
SULFOXIDES
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS
TRITIUM COMPOUNDS
VASCULAR DISEASES
VERTEBRATES
VITAMIN E
VITAMINS