Xanthine oxidase inhibition attenuates ischemic-reperfusion lung injury

Journal Article · Sun May 01 04:00:00 EDT 1988 · J. Surg. Res.; (United States)

DOI:https://doi.org/10.1016/0022-4804(88)90159-X· OSTI ID:6971780

Lynch, M J; Grum, C M; Gallagher, K P; Bolling, S F; Deeb, G M; Morganroth, M L

Ischemic-reperfusion lung injury is a factor potentially limiting the usefulness of distant organ procurement for heart-lung transplantation. Toxic oxygen metabolites are considered a major etiologic factor in reperfusion injury. Although oxygen-free radicals may be generated by many mechanisms, we investigated the role of xanthine oxidase in this injury process by using lodoxamide, a xanthine oxidase inhibitor, to inhibit ischemic-reperfusion injury in an isolated rat lung model. Isolated rat lungs were perfused with physiologic salt solution (PSS) osmotically stabilized with Ficoll until circulating blood elements were nondetectable in the pulmonary venous effluent. Lungs were rendered ischemic by interrupting ventilation and perfusion for 2 hr at 37/sup 0/C. After the ischemic interval, the lungs were reperfused with whole blood and lung injury was determined by measuring the accumulation of /sup 125/I-bovine serum albumin in lung parenchyma and alveolar lavage fluid as well as by gravimetric measurements. Lung effluent was collected immediately pre- and postischemia for analysis of uric acid by high-pressure liquid chromatography. Lodoxamide (1 mM) caused significant attenuation of postischemic lung injury. Uric acid levels in the lung effluent confirmed inhibition of xanthine oxidase. Protection from injury was not complete, however, implying that additional mechanisms may contribute to ischemic-reperfusion injury in the lung.

• Research Organization: Univ. of Michigan Hospitals, Ann Arbor (USA)
• OSTI ID: 6971780
• Journal Information: J. Surg. Res.; (United States), Journal Name: J. Surg. Res.; (United States) Vol. 44:5; ISSN JSGRA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMALS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BLOOD FLOW
BODY
CARDIOVASCULAR DISEASES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYME INHIBITORS
ENZYMES
ETIOLOGY
HETEROCYCLIC COMPOUNDS
INHIBITION
INJURIES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISCHEMIA
ISOTOPE APPLICATIONS
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
MAMMALS
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGEN
PERFUSED ORGANS
PROTEINS
PURINES
RADICALS
RADIOISOTOPES
RATS
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
URIC ACID
VASCULAR DISEASES
VERTEBRATES
XANTHINES