Neutrophil accumulation in ischemic canine myocardium. Insights into time course, distribution, and mechanism of localization during early reperfusion

Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Circulation; (United States)

DOI:https://doi.org/10.1161/01.CIR.84.1.400· OSTI ID:5449345

Dreyer, W J; Michael, L H; West, M S; Smith, C W; Rothlein, R; Rossen, R D; Anderson, D C; Entman, M L ^[1]

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1. Section of Cardiovascular Sciences, Methodist Hospital, Houston, TX (USA)

The authors have previously demonstrated that chemotactic factors released from the ischemic canine myocardium peak early during reperfusion and that they elicit neutrophil adherence reactions in vitro that are dependent on the CD18 glycoprotein family. In this study they investigated the hypothesis that neutrophil localization in ischemic canine myocardium in vivo occurs over a similar time course during early reperfusion and involves a CD18-dependent mechanism. they concluded the circumflex coronary artery for 1 hour in acute, open-chest dogs, followed by reperfusion for 1, 2, 3, or 4 hours. Regional myocardial blood flow was determined using radiolabeled microspheres, and localization was traced using technetium-99m-labeled autologous neutrophils. In the first hour of reperfusion, neutrophil localization occurred preferentially within the subendocardial region and was inversely related to flow. Neutrophil localization diminished across the ischemic myocardium from endocardium to epicardium but remained negatively related to flow in the midmyocardial region. Regardless of flow, little neutrophil localization occurred in the subepicardial region. Neutrophil localization was greatest in the first hour of reperfusion and diminished thereafter. By 4 hours of reperfusion, the rate of localization was markedly attenuated relative to 1 hour. Dogs given anti-CD18 monoclonal antibody R15.7 (1 mg/kg i.v.) before occlusion underwent 1 hour of occlusion followed by 1 hour of reperfusion. When compared with 1-hour reperfusion controls, the R15.7-treated dogs demonstrated significant attenuation of neutrophil localization in the subendocardial region. These data support the concepts that rapid neutrophil localization during reperfusion occurs within regions of previous myocardial ischemia and that neutrophils preferentially localize within the subendocardial region.

• OSTI ID: 5449345
• Journal Information: Circulation; (United States), Journal Name: Circulation; (United States) Vol. 84:1; ISSN 0009-7322; ISSN CIRCA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANEMIAS
ANIMALS
ANTIBODIES
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD FLOW
BLOOD VESSELS
BODY
BODY FLUIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CORONARIES
DISEASES
DOGS
HEART
HEMIC DISEASES
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISCHEMIA
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MICROSPHERES
MONOCLONAL ANTIBODIES
MUSCLES
MYOCARDIUM
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PATHOGENESIS
PERFUSED ORGANS
RADIOISOTOPES
SYMPTOMS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOI