Substrate use in ischemic and reperfused canine myocardium: quantitative considerations

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:6148135

Myears, D W; Sobel, B E; Bergmann, S R

The patterns of use of substrate in reperfused myocardium are not yet well elucidated, and their delineation is essential for adequate interpretation of results of analyses performed after positron emission tomography with labeled substrates to differentiate normal from abnormal heart muscle. Accordingly, in open-chest, anesthetized dogs the authors measured glucose and fatty acid utilization in normal, ischemic, and reperfused myocardium and assessed the contributions of metabolism of each substrate to overall oxidative metabolism. Intracoronary (/sup 3/H)glucose and (/sup 14/C)palmitate were administered in five control dogs, eight dogs subjected to 1 h of coronary occlusion, and nine dogs subjected to reperfusion after 1 h of ischemia. Regional coronary venous blood samples were assayed sequentially. In reperfused myocardium, utilization of glucose was 103% greater than that in ischemic and 273% greater than in normal myocardium. Utilization of fatty acid during reperfusion, although greater than that in ischemic myocardium, was significantly less than that in normal myocardium despite restoration of flow to 80% of control values. Despite diminished net uptake of fatty acid, oxidation of fatty acid accounted for 63% of total oxygen consumption in reperfused myocardium. These studies indicate that canine myocardium reperfused after 1 h of ischemia exhibits enhanced uptake of glucose and impaired utilization of palmitate. Nevertheless, palmitate continues to comprise the substrate primarily utilized for oxidative metabolism.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO (USA)
• OSTI ID: 6148135
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 253:1; ISSN AJPHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ALKALINE EARTH ISOTOPES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BLOOD FLOW
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CERIUM 141
CERIUM ISOTOPES
COMPUTERIZED TOMOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
EMISSION COMPUTED TOMOGRAPHY
EVEN-ODD NUCLEI
FLUORINE 18
FLUORINE ISOTOPES
GLUCOSE
HEART
HEXADECANOIC ACID
HEXOSES
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISCHEMIA
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
MUSCLES
MYOCARDIUM
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PERFUSED TISSUES
POSITRON COMPUTED TOMOGRAPHY
RADIOISOTOPES
RARE EARTH ISOTOPES
RARE EARTH NUCLEI
SACCHARIDES
SCANDIUM 46
SCANDIUM ISOTOPES
SECONDS LIVING RADIOISOTOPES
STRONTIUM 85
STRONTIUM ISOTOPES
SUBSTRATES
TISSUES
TOMOGRAPHY
TRITIUM COMPOUNDS
UPTAKE
VASCULAR DISEASES
VERTEBRATES