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Metabolic tissue characterization of reperfused canine myocardium by C-11 palmitic acid

Technical Report ·
OSTI ID:5349233

While oxidation of fatty acid declines or even ceases during ischemia, the recovery of myocardial fatty acid metabolism after transient ischemia has remained largely unexplored. Using C-11 palmitic acid as a tracer of myocardial fatty acid metabolism, we evaluated in open chest dogs the myocardial retention and subsequent tissue clearance of intracoronary injected C-11 palmitic acid at control and up to 3 hours after a 20 min occlusion of the LAD. In animals with permanent occlusion, the relative size and halftime of the early clearance curve component, representing oxidation of C-11 palmitic acid, differed markedly from control and did not change with ongoing ischemia. In animals with a 20 min coronary occlusion, the relative size of the early C-11 clearance phase was still significantly depressed at 20 and 90 min of reperfusion but had returned to control at 180 min. The tissue C-11 clearance halftimes remained significantly prolonged throughout the entire reperfusion period. Regional function in the reperfused segments monitored with ultrasonic crystals recovered only slowly and was still less than control after 3 hrs of reperfusion. These data indicate that myocardial fatty acid metabolism after transient ishemia recovers slowly. C-11 palmitic acid in conjunction with Positron-CT may thus be useful for studying regional fatty acid metabolism noninvasively following an ischemic injury and may be helpful in identifying reversible tissue injury.

Research Organization:
California Univ., Los Angeles (USA)
DOE Contract Number:
AC03-76SF00012
OSTI ID:
5349233
Report Number(s):
DOE/SF/00012-T21; ON: DE84005633
Country of Publication:
United States
Language:
English