Spacial and temporal profiles of neutrophil accumulation in the reperfused ischemic myocardium

Journal Article · Mon Jan 01 04:00:00 EST 1990 · American Journal of Cardiovascular Pathology; (USA)

OSTI ID:6212186

de Lorgeril, M; Rousseau, G; Basmadjian, A; St-Jean, G; Tran, D C; Latour, J G ^[1]

---

1. Montreal Heart Institute, Quebec (Canada)

To elucidate further the pathogenic role of neutrophils in evolving reperfused myocardial infarction, we investigated the dynamics of their accumulation and distribution in the ischemic myocardium. The left anterior descending coronary artery was occluded in dogs for 2 hours followed by reperfusion for 0, 3, 6, or 24 hours. 111In-labeled neutrophils were injected at the time of occlusion or after 16 hours of reperfusion. The area at risk was similar among groups. Infarct size expressed in percent of the area at risk was identical between groups reperfused for 6 (35.2 +/- 4.4%) or 24 (32.3 +/- 3.9%) hours but smaller (22.0 +/- 4.4%; p less than 0.05) after 3 hours of reperfusion. 111In-neutrophils accumulation quantified by scintigraphy correlated positively with infarct size (r = 0.64, p less than 0.005); accumulation rates (cells/h/cm2MI) were high during the first 3 (2288 +/- 754) and 6 hours (1953 +/- 463) but low (490 +/- 192) between 16 and 24 hours of reperfusion. Cells accumulating during reperfusion (12,566 +/- 2307 cells/g at 3 hours) were found within the borders of the necrotic area, and the cell counts (2420 +/- 724 cells/g, p less than 0.05) in the live tissue located within the area at risk after 3 hours of reperfusion were similar to those found in the subepicardium at the onset of reperfusion: (2240 +/- 571 cells/g). Only a few cells were detected in the normally perfused myocardium (67 +/- 33 cells/g). We conclude that reperfusion accumulation in the ischemic myocardium; the reaction takes place within 3-6 hours of reperfusion, a period of time where infarct size is growing by about 40%. These results support the concept that leukocytes may play a pathogenic role on infarct size in models with brief ischemia followed by reperfusion.

• OSTI ID: 6212186
• Journal Information: American Journal of Cardiovascular Pathology; (USA), Journal Name: American Journal of Cardiovascular Pathology; (USA) Vol. 3:2; ISSN AJCPE; ISSN 0887-8005
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
DISTRIBUTION
DOGS
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
HEART
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISCHEMIA
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MUSCLES
MYOCARDIAL INFARCTION
MYOCARDIUM
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PATHOGENESIS
RADICALS
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES