Comparison of cytochromes from anaerobically and aerobically grown cells of Pseudomonas perfectomarinus

Journal Article · Fri Apr 01 04:00:00 EST 1983 · J. Bacteriol.; (United States)

OSTI ID:6057618

Liu, M C; Payne, W J; Peck, Jr, H D; LeGall, J

Pseudomonas perfectomarinus (ATCC 14405) is a facultative anaerobe capable of either oxygen respiration or anaerobic nitrate respiration, i.e., denitrification. A comparative study of the electron transfer components of cells revealed five c-type cytochromes and cytochrome cd in the soluble fraction from anaerobically grown cells and four c-type cytochromes in the soluble fraction from anaerobically grown cells. Purification procedures yielded three c-type cytochromes (designated c-551, c-554, and acidic c-type) from both kinds of cells as indicated by similarities in absorption spectra, molecular weight, and electrophoretic mobility. Cytochrome cd, a diheme c-type cytochrome (cytochrome c-552), and a spilt-..cap alpha..-c-type cytochrome with a low ratio of ..cap alpha.. to ..beta.. absorption peak heights was uniquely present in the aerobically grown cells. Liquid N/sub 2/ temperature absorption spectroscopy on the membrane fraction from anaerobically grown cells revealed residual cytochrome cd as well as differences in the relative amounts of c-type and b-type cytochromes in membranes prepared from cells grown under the two different conditions. 28 references, 3 figures, 3 tables.

• Research Organization: Univ. of Georgia, Athens
• OSTI ID: 6057618
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 154:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTRA
AEROBIC CONDITIONS
ANAEROBIC CONDITIONS
BACTERIA
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
CYTOCHROMES
DATA
DENITRIFICATION
ELECTRON TRANSFER
EXPERIMENTAL DATA
INFORMATION
MEMBRANES
MICROORGANISMS
MOLECULAR WEIGHT
NUMERICAL DATA
PIGMENTS
PSEUDOMONAS
PURIFICATION
SPECTRA