Cytochrome components of nitrate- and sulfate-respiring Desulfovibrio desulfuricans ATCC 27774

Journal Article · Wed Nov 30 23:00:00 EST 1988 · Journal of Bacteriology; (USA)

OSTI ID:6939409

Liu, M C; LeGall, J ^[1]; Costa, C; Coutinho, I B; Moura, J J.G.; Moura, I; Xavier, A V ^[2]

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1. Univ. of Georgia, Athens (USA)
2. New Univ. of Lisbon (Portugal)

Three multiheme c-type cytochromes - the tetraheme cytochrome c{sub 3} (molecular weight (MW) 13,500), a dodecaheme cytochrome c (MW 40,800), and a split-Soret cytochrome c (MW 51,540), which is a dimer with 2 hemes per subunit (MW 26,300) - were isolated from the soluble fraction of Desulfovibrio desulfuricans (ATCC 27774) grown under nitrate- or sulfate-respiring conditions. Two of them, the dodecaheme and the split-Soret cytochromes, showed no similarities to any of the c-type cytochromes isolated from other sulfate-reducing bacteria, while the tetraheme cytochrome c{sub 3} appeared to be analogous to the cytochrome c{sub 3} found in other sulfate-reducing bacteria. For all three multiheme c-type cytochromes isolated, the homologous proteins from nitrate- and sulfate-grown cells were indistinguishable in amino acid composition, physical properties, and spectroscopic characteristics. It therefore appears that the same c-type cytochrome components are present when D. desulfuricans ATCC 27774 cells are grown under either condition. This is in contrast to the considerable difference found in Pseudomonas perfectomarina, a marine denitrifier, when the cells are grown on nitrate or oxygen as the terminal electron acceptor. In addition, two spectroscopy methods capable of revealing minute structural variations in proteins provided identical information about the tetraheme cytochrome c{sub 3} from nitrate-grown and sulfate-grown cells.

• OSTI ID: 6939409
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 170:12; ISSN 0021-9193; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CARBOXYLIC ACIDS
CLASSIFICATION
CYTOCHROMES
DESULFOVIBRIO
HEME
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
METABOLISM
MICROORGANISMS
NITRATES
NITROGEN COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
SULFATE-REDUCING BACTERIA
SULFATES
SULFUR COMPOUNDS