New electrophoretic method for determining ligand: technetium stoichiometry in carrier free /sup 99m/Tc-radiopharmaceuticals

Journal Article · Wed Aug 01 04:00:00 EDT 1979 · J. Nucl. Med.; (United States)

OSTI ID:6057580

Marzilli, L G; Worley, P; Burns, H D

An electrophoretic procedure is outlined for the determination of the number of ligands bound to technetium-99m radiopharmaceuticals. The approach involves use of ligands that will complex technetium in a similar fashion but that differ in charge. This approach was applied experimentally to dimercapto ligands in which the ligating sulfur atoms are separated by a flexible three-carbon chain (1,3-dimercapto compounds). Two such ligands studied are 1,3-dimercaptopropane (DMP) and dihydrothioctic acid (DHTA). The Tc compound of DHTA migrates much farther on electrophoresis than the Tc complex of DMP. However, when TcO/sub 4//sup -/ is reduced by SnCl/sub 2/ or NaBH/sub 4/ in the presence of equimolar quantities of DHTA and DMP, a new compound is formed being twice as abundant as either the TcDMP or the TcDHTA compound and migrating an intermediate distance. The formation of this new complex and the 1:2:1 distribution indicates that two 1,3-dimercapto compounds are attached to the Tc-center in all three compounds.

• Research Organization: Johns Hopkins Univ., Baltimore, MD
• OSTI ID: 6057580
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 20:8; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550200 -- Biochemistry
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACIDS
CHELATING AGENTS
COMPARATIVE EVALUATIONS
DATA
DATA FORMS
DISULFIDES
DRUGS
ELECTROPHORESIS
ESTERS
EXPERIMENTAL DATA
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HOURS LIVING RADIOISOTOPES
HYDROXY ACIDS
INFORMATION
INTERMEDIATE MASS NUCLEI
ISOLATED VALUES
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGANDS
LIPOTROPIC FACTORS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PERTECHNETATES
RADIOACTIVE MATERIALS
RADIOISOTOPES
RADIOPHARMACEUTICALS
STEREOCHEMISTRY
TECHNETIUM 99
TECHNETIUM COMPOUNDS
TECHNETIUM ISOTOPES
THIOCTIC ACID
THIOLS
TRANSITION ELEMENT COMPOUNDS
YEARS LIVING RADIOISOTOPES