Effects of tumor promoters and steroidal anti-inflammatory agents on skin of newborn mice in vivo and in vitro
- Oak Ridge National Lab., TN
The phorbol esters 12-O-tetradecanoylphorbol-13-acetate (TPA) and 12-O-hexadecanoylphorbol-13 acetate (HPA) are not only potent tumor promoters but also potent stimulators of epidermal DNA synthesis and ornithine decarboxylase (ODC) activity in adult mice. However, when applied topically to newborn mice, TPA and HPA have essentially no effect on epidermal and dermal DNA synthesis or on epidermal ODC activity. Exposure of primary cultures of newborn mouse epidermal cells to TPA or HPA markedly stimulated both DNA synthesis and ODC activity. The anti-inflammatory steroids dexamethasone and fluocinolone acetonide (FA) were found to be potent inhibitors of tumor promotion and epidermal DNA synthesis in adult mice. However, when applied topically to newborn mice, FA did not inhibit epidermal or dermal DNA synthesis but stimulated it approximately twofold at 48 hours after FA treatment. In primary cultures of epidermal cells from newborn mice, treatment with dexamethasone or FA caused an early stimulation of DNA synthesis followed by a 50% inhibition of DNA synthesis 2 to 3 days after a 1-hour pulse treatment. Also, DNA synthesis was moderately inhibited when FA was added to primary cultures of dermal fibroblasts. In their reaction to tumor promoters, epidermal cells in culture behaved more like adult than newborn mouse epidermis in vivo, whereas anti-inflammatory steroids gave an intermediate response.
- OSTI ID:
- 6045752
- Journal Information:
- J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 60:2; ISSN JNCIA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effects of antiinflammatory agents on mouse skin tumor promotion, epidermal DNA synthesis, phorbol ester-induced cellular proliferation, and production of plasminogen activator. [Fluocinolone acetonide, fluocinonide, fluclorolone acetonide]
Differential retinoic acid inhibition of ornithine decarboxylase induction by 12-O-tetradecanoylphorbol-13-acetate and by germicidal ultraviolet light
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AGE DEPENDENCE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BODY
CARBOXYLIC ACIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
DECARBOXYLASE
DEXAMETHASONE
DISEASES
DNA REPLICATION
ENZYMES
EPIDERMIS
EPITHELIUM
FIBROBLASTS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
INFLAMMATION
KETONES
LYASES
MAMMALS
MICE
NEOPLASMS
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
ORNITHINE
PATHOLOGICAL CHANGES
PREGNANES
PROMOTERS
RODENTS
SKIN
SOMATIC CELLS
STEROID HORMONES
STEROIDS
TISSUES
VERTEBRATES