Regional differences in adipocyte lactate production from glucose

Journal Article · Tue Nov 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6038848

Newby, F D; Sykes, M N; DiGirolamo, M ^[1]

---

1. Emory Univ. School of Medicine, Atlanta, GA (USA)

Having shown that lactate is an important product of glucose metabolism by rat epididymal adipocytes, the authors investigated possible regional differences in adipocyte lactate production and the role of the animals' nutritional state and stage of development. (U-{sup 14}C)glucose metabolism, lactate production, and response to insulin were measured in fat cells isolated from four adipose regions from young lean and older fatter rats, killed either in the fed state or after fasting for 48 h. In the absence of insulin, mesenteric fat cells from either age group metabolized significantly more glucose per cell and converted more glucose to lactate than cells from other depots, regardless of nutritional state. Adipocytes from fasted lean rats showed a significant increase in the relative glucose conversion to lactate in all depots when compared with cells from fed lean rats. Fasting of older fatter rats, however, had limited effects on the relative adipocyte glucose conversion to lactate since lactate production was already high. Mesenteric fat cells had the lowest relative response to insulin, possibly due to the high basal rate of glucose metabolism. These findings indicate that differences exist among adipose regions in the rates of glucose metabolism, lactate production and response to insulin. The anatomical location of the mesenteric adipose depot, coupled with a high metabolic rate and blood perfusion, suggests that mesenteric adipocytes may provide a unique and more direct contribution of metabolic substrates for hepatic metabolism than adipocytes from other depots.

• OSTI ID: 6038848
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALDEHYDES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOCHEMISTRY
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMISTRY
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
FASTING
FAT CELLS
GLUCOSE
HEXOSES
HORMONES
HYDROXY ACIDS
INSULIN
LABELLED COMPOUNDS
LACTIC ACID
MAMMALS
METABOLISM
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
TISSUES
VERTEBRATES