Thiophene metabolism by E. coli

Technical Report · Tue Jan 01 04:00:00 EST 1991

DOI:https://doi.org/10.2172/6026023· OSTI ID:6026023

Clark, D P

The objective of this project is to investigate the mechanism of degradation of sulfur-containing heterocyclic molecules by mutant strains of Escherichia coli K-12. We have previously isolated multiple mutants of E. coli which had gained the capacity to oxidize thiophene compounds and their furan analogs. We have focused on the thdA mutation in our subsequent research, as this appears to be in a regulatory gene central to the thiophene/furan oxidation system. The thdF gene appears to be more directly involved in the oxidation reactions, whereas thdC and thdD are apparently required for increased protection against the toxic effects of thiophene and furan compounds. 4 tabs.

• Research Organization: Southern Illinois Univ., Carbondale, IL (United States). Dept. of Microbiology
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG22-87PC79912
• OSTI ID: 6026023
• Report Number(s): DOE/PC/79912-14; ON: DE92002766
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AROMATICS
AZAARENES
AZOLES
BACTERIA
BIODEGRADATION
BIOTECHNOLOGY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECOMPOSITION
DESULFURIZATION
DNA POLYMERASES
DOCUMENT TYPES
ENZYMES
ESCHERICHIA COLI
GENE MUTATIONS
GENETIC ENGINEERING
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INDOLES
METABOLISM
MICROORGANISMS
MUTATIONS
NUCLEOTIDYLTRANSFERASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDATION
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROGRESS REPORT
PROTEINS
PYRROLES
THIOPHENE
TRANSFERASES
TRYPTOPHAN