Choline incorporation by Schistosoma mansoni: distribution of choline metabolites during development and after sexual differentiation

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · J. Parasitol.; (United States)

DOI:https://doi.org/10.2307/3282131· OSTI ID:6025411

Ancelin, M L; Torpier, G; Vial, H J; Capron, A

Choline metabolism was investigated in Schistosoma mansoni during the main phases of its development, namely, schistosomula, 11- and 15-day-old worms, and adults. At the physiological choline concentration used in the assay (20 microM), betaine was, along with phosphatidylcholine, one of the most abundant choline metabolites, revealing considerable choline oxidation activity. Very little radioactivity was associated with CDP-choline, whereas a sustained incorporation into phosphocholine occurred. These results provide good evidence that CTP:phosphocholine cytidylyltransferase plays a regulatory role in the de novo pathway of phosphatidylcholine biosynthesis. During development, the incorporation of choline into its various metabolites was maximal in 11-day-old worms. At this stage, the oxidative pathway predominated over the Kennedy pathway, whereas at all other stages the de novo phosphatidylcholine biosynthesis was predominant. Furthermore, choline incorporation into betaine was much more important in the adult female worm than in the male, indicating a major difference in choline incorporation and distribution between the 2 sexes of the adult worms.

• Research Organization: INSERM U.58--CNRS UA 530, Montpellier, France
• OSTI ID: 6025411
• Journal Information: J. Parasitol.; (United States), Journal Name: J. Parasitol.; (United States) Vol. 73:3; ISSN JOPAA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
BETAINE
BIOLOGICAL PATHWAYS
CARBOXYLIC ACIDS
CELL DIFFERENTIATION
CHOLINE
DRUGS
ENZYME ACTIVITY
ENZYMES
HELMINTHS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIPOTROPIC FACTORS
METABOLISM
METABOLITES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLATYHELMINTHS
QUATERNARY COMPOUNDS
RADIOISOTOPES
SCHISTOSOMA
SEX DEPENDENCE
TRACER TECHNIQUES
TRANSFERASES
TREMATODES