Effect of lead ingestion on superoxide dismutase activity in rats

Conference · Wed Jan 01 04:00:00 EST 1986 · Trace Subst. Environ. Health; (United States)

OSTI ID:6014052

Mylroie, A A; Boseman, A; Kyle, J

The authors have reported previously that Pb exposure in rats resulted in reduced levels of Cu in blood and a reduction in the activities of the Cu dependent enzymes ceruloplasmin in serum and superoxide dismutase (CuSOD) in erythrocytes. They have now determined the effect of Pb in rats on levels of Cu in tissue and on the activities of CuSOD and MnSOD (Mn dependent SOD). Groups of 24-26 day old rats were fed a nutritionally adequate purified diet and given lead acetate in the drinking water for five weeks. At the end of the experimental period, blood and excised tissue were wet ashed with concentrated HNO/sub 3/ and subsequently analyzed by atomic absorption spectrophotometry. SOD activity was assayed by the increase in the rate of the riboflavin sensitized photo-oxidation and o-dianisidine in the absence and presence of cyanide (CN/sup -/). Lead exposure resulted in decreased Cu content of tissue with a concomitant reduction in CuSOD (CN/sup -/ sensitive) activity in liver, kidney and heart. Total SOD activity (CuSOD and MnSOD) was reduced in liver, but not in heart and kidney. In heart and kidney, the reduction in CuSOD appears to be compensated by an increase in MnSOD. The induction of MnSOD activity may be the result of an increase in superoxide anion concentration owing to an increased production or a decreased rate of removal of superoxide radical anions (O/sub 2//sup -/).

• Research Organization: Chicago State Univ., IL
• OSTI ID: 6014052
• Report Number(s): CONF-860626-
• Conference Information: Journal Name: Trace Subst. Environ. Health; (United States)
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION SPECTROSCOPY
ANIMALS
ANIONS
BODY
CARDIOVASCULAR SYSTEM
CHARGED PARTICLES
DATA
DIGESTIVE SYSTEM
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
GLANDS
HEART
INFORMATION
INGESTION
INTAKE
IONS
KIDNEYS
LEAD
LIVER
MAMMALS
METALS
NUMERICAL DATA
ORGANS
OXIDOREDUCTASES
RADICALS
RATS
RODENTS
SPECTROSCOPY
SUPEROXIDE DISMUTASE
TOXICITY
VERTEBRATES