Simplified approach to confidence limits in radioimmunoassay
A simple method of calculating confidence limits for radioimmunoassay data is presented. The method involves the use of the within-assay variation in dose estimate of three routine quality-control specimens, measured in repeated assays, to estimate the confidence limits for results on unknown samples. Results for control specimens are combined by calculating the unique quadratic curve fitting a graph of within-assay standard deviation vs mean value for each control. This method requires no special data accumulation or advanced computing equipment. For cortisol, lutropin, and thyroxine radioimmunassays, confidence limits calculated in this way have been compared with three calculated from the variance of the response variable B/Bq in repeated standard curves. Both methods agree well with actual limits observed when plasma pools containing a wide range of hormone concentrations are assayed repeatedly.
- Research Organization:
- Royal Prince Alfred Hospital, Camperdown, Australia
- OSTI ID:
- 6012221
- Journal Information:
- Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 26:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
RADIOIMMUNOASSAY
QUALITY CONTROL
CALCULATION METHODS
HORMONES
HYDROCORTISONE
STATISTICS
THYROXINE
VARIATIONS
ADRENAL HORMONES
AMINO ACIDS
CARBOXYLIC ACIDS
CONTROL
CORTICOSTEROIDS
GLUCOCORTICOIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
MATHEMATICS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PEPTIDE HORMONES
PREGNANES
RADIOASSAY
STEROID HORMONES
STEROIDS
THYROID HORMONES
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics