Internal sample attenuator counting (ISAC). A new technique for separating and measuring bound and free activity in radioimmunoassays
Journal Article
·
· Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:5799416
A new method for the separation counting of bound and free activity in radioimmunoassays is described. Particles containing a radiation-abosrbing (attenuating) material are added to the assay. They shield the radiation from either the antibody-bound or the free radioligand. This obviates such manipulations conventionally involved in the separation and counting steps of radioimmunoassays as centrifugation decanting. Bismuth oxide is used as the attenuator. Particles with different properties are described. In one type, bismuth oxide is combined with active charcoal in an agarose matrix and serves as an absorbant for the free radioligand. In another type bismuth oxide is trapped within a polyacrylamide matrix to which antibodies are coupled. This particle can be used with a first- or a second-antibody bound activity. Application of the technique is illustrated with radioimmunoassays for thyroxin, triiodothyronine, human choriogonadotropin, and lutropin (luteinizing hormone).
- Research Organization:
- Univ. of Lund at Malmo General Hospital, Sweden
- OSTI ID:
- 5799416
- Journal Information:
- Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:12; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BISMUTH COMPOUNDS
BISMUTH OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
COMPARATIVE EVALUATIONS
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GONADOTROPINS
HCG
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LH
LIGANDS
MEASURING METHODS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIATION PROTECTION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
THYROID HORMONES
THYROXINE
TRACER TECHNIQUES
TRIIODOTHYRONINE
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BISMUTH COMPOUNDS
BISMUTH OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
COMPARATIVE EVALUATIONS
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GONADOTROPINS
HCG
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LH
LIGANDS
MEASURING METHODS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIATION PROTECTION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
THYROID HORMONES
THYROXINE
TRACER TECHNIQUES
TRIIODOTHYRONINE