Impaired mitochondrial metabolism and protein synthesis in streptozotocin diabetic rat hepatocytes

Conference · Sun Feb 25 23:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6010908

Memon, R A; Bessman, S P; Mohan, C ^[1]

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1. Univ. of Southern California, Los Angeles (United States)

Isolated hepatocytes prepared from control, streptozotocin diabetic rats were incubated at 30{degrees}C in Krebs-Henseleit bicarbonate buffer, pH 7.4, containing 0.5 mM concentration of each of the 20 natural amino acids. Effect of insulin on the oxidation of 2,3-{sup 14}C and 1,4-{sup 14}C succinate (suc) carbons and their incorporation into hepatocyte protein, lipid and various metabolic intermediates was studied. Mitochondrial oxidation of suc carbons and their incorporation into protein and lipid was significantly lower in diabetic and insulin treated diabetic rats. Diabetic rats failed to exhibit any significant insulin effect on the oxidation of either 2,3 or 1,4-{sup 14}C suc carbons. Amphibolic channeling of 2,3-{sup 14}C suc carbons into amino acids was significantly reduced in hepatocytes of diabetic rats, however, more of these carbons were diverted into the gluconeogenesis pathway. Diabetes caused a far greater decrease in the oxidation of 2,3-{sup 14}C suc carbons as compared to 1,4-{sup 14}C suc. Based on an earlier report that insulin stimulates only the intramitochondrial Krebs cycle reactions, the authors conclude that the diminished level of anabolic activities in the diabetic rat hepatocytes is due to the subsequent reduction in amphibolic channeling of metabolic intermediates.

• OSTI ID: 6010908
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 4:3; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DIABETES MELLITUS
DISEASES
ENDOCRINE DISEASES
HORMONES
INSULIN
ISOTOPE APPLICATIONS
KREBS CYCLE
LABELLED COMPOUNDS
LIPIDS
LIVER CELLS
MAMMALS
METABOLIC DISEASES
METABOLISM
MITOCHONDRIA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RATS
REACTION INTERMEDIATES
RODENTS
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES