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Intracellular site of insulin action - the mitochondrial Krebs cycle

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7246470
;

Effect of insulin on the oxidation of carbons 1,4 and 2,3 of succinate and their incorporation into protein were studied in isolated hepatocytes of rat. Oxidation of carbons 2,3 (aliphatic carbons) of succinate and their incorporation into protein were significantly stimulated by insulin. Insulin had only a trivial effect on the formation of /sup 14/CO/sub 2/ from carbons 1,4 of succinate. Insulin effect on the oxidation of carbons 2,3 of succinate was a rapid one. Within 2 minutes following the isotope addition most of the radioactivity was recovered in the amino acid fraction. Stimulation of /sup 14/CO/sub 2/ formation from the aliphatic carbons of succinate was about three times as much as from the carboxyl carbons, but much more net /sup 14/CO/sub 2/ formation occurred from the carboxyl groups which are oxidized either outside the mitochondrial Krebs cycle or do not complete one full pass in the Krebs cycle. These results show that insulin acts on a small compartment, the mitochondrial Krebs cycle, which is directly coupled to the anabolic utilization of energy.

Research Organization:
Univ. of Southern California School of Medicine, Los Angeles
OSTI ID:
7246470
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHALCOGENIDES
DICARBOXYLIC ACIDS
HORMONES
INSULIN
ISOTOPE APPLICATIONS
KINETICS
KREBS CYCLE
LABELLED COMPOUNDS
LIVER CELLS
MAMMALS
METABOLISM
MITOCHONDRIA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RATS
REACTION KINETICS
RODENTS
SOMATIC CELLS
SUCCINIC ACID
TRACER TECHNIQUES
VERTEBRATES