Extracellular phosphate requirement for insulin action on isolated rat hepatocytes

Conference · Mon Feb 26 04:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6057110

Rogers, K S; Memon, R A; Mohan, C; Gieger, P; Bessman, S P ^[1]

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1. Medical Coll. of Virginia, Richmond (United States) Univ. of Southern California, Los Angeles (United States)

Isolated rat hepatocytes were prepared in Krebs-Henseleit bicarbonate (KHB) buffer, pH 7.4, by a procedure described earlier. They were centrifuged and washed twice in KHB buffer containing 0, 0.05, 0.1, 0.02, 0.05, 0.89 or 1.0 mM phosphate and were incubated at 30{degrees}C in the presence of tracer 2,3-{sup 14}C-succinate and 0.5 mM concentration of each of the 20 natural amino acids. Hepatocytes washed and incubated in KHB buffer containing less than 0.05 mM phosphate failed to show any insulin stimulation of 2,3-{sup 14}C-succinate oxidation or protein incorporation of tracer carbons. Mitochondrial oxidation of succinate carbons was significantly lower in the medium containing less than 1.0 mM phosphate. Maximal insulin stimulatory effect was observed in the presence of 1 mM phosphate in the medium. These data indicate that a diminished insulin responsiveness in hypophosphatemic patients may be due to the insensitivity of mitochondria to insulin in the hypophosphatemic state.

• OSTI ID: 6057110
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DICARBOXYLIC ACIDS
HORMONES
INSULIN
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIVER CELLS
MAMMALS
METABOLISM
MITOCHONDRIA
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PHOSPHATES
PHOSPHORUS COMPOUNDS
PROTEINS
RATS
REACTION KINETICS
RODENTS
SOMATIC CELLS
SUCCINIC ACID
TRACER TECHNIQUES
UPTAKE
VERTEBRATES