Concentration-dependent mutation of diploid human lymphoblasts by methylnitronitrosoguanidine: the importance of phenotypic lag

Journal Article · Wed Dec 31 23:00:00 EST 1975 · Somatic Cell Genet.; (United States)

DOI:https://doi.org/10.1007/BF01538837· OSTI ID:6010827

Penman, B W; Thilly, W G

The mutation of diploid human lymphoblasts by methylnitronitrosoguanidine (MNNG) was measured over the range of 0 to 45 ng of MNNG/ml of medium. We found a 12-day lag in the phenotypic expression of 6-thioguanine resistance; the occurrence of this lag was independent of MNNG concentration. We hypothesize that the unexpectedly long lag period reflects a requirement for the loss of previously existing molecules of the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT) after mutation at the HGPRT locus.

• Research Organization: Massachusetts Inst. of Tech., Cambridge
• OSTI ID: 6010827
• Journal Information: Somatic Cell Genet.; (United States), Journal Name: Somatic Cell Genet.; (United States) Vol. 2:4; ISSN SCEGD
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBONIC ACID DERIVATIVES
CHEMISTRY
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
ENZYMES
GENETIC EFFECTS
GLYCOSYL TRANSFERASES
GUANIDINES
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MUTATIONS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PENTOSYL TRANSFERASES
PHENOTYPE
PRIMATES
SOMATIC CELLS
TRANSFERASES
VERTEBRATES