Quantitative assay for mutation in diploid human lymphoblasts using microtiter plates

Journal Article · Wed Dec 31 23:00:00 EST 1980 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(81)90103-2· OSTI ID:6498273

Furth, E A; Thilly, W G; Penman, B W; Liber, H L; Rand, W M

A microtiter plating technique which eliminates the need for soft agar and fibroblast feeder layers to determine the colony-forming ability of diploid human lymphoblast lines was described. The calculation of cloning efficiency is based on the Poisson distribution, and a statistical method for calculating confidence intervals is presented. This technique has been applied to the comcomitant examination of induced mutation at the putative loci for hypoxanthine guanine phosphoribosyl transferase, thymidine, kinase, and Na/sup +//K/sup +/ adenosine triphosphatase.

• Research Organization: Massachusetts Inst. of Tech., Cambridge
• OSTI ID: 6498273
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 110:1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BINDING ENERGY
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CULTURES
CELL DIVISION
CHARGED PARTICLES
CLONE CELLS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DRUGS
ENERGY
ENZYME ACTIVITY
ENZYMES
ESTERS
GENE MUTATIONS
GENETIC EFFECTS
GLYCOSIDES
HETEROCYCLIC COMPOUNDS
IONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MATHEMATICS
MUTAGEN SCREENING
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OUABAIN
POTASSIUM IONS
PRIMATES
PYRIMIDINES
QUANTITY RATIO
RIBOSIDES
SCREENING
SODIUM IONS
SOMATIC CELLS
STATISTICS
STROPHANTHINS
SULFONIC ACID ESTERS
THYMIDINE
TIME DEPENDENCE
VERTEBRATES