Fragmentation of gelatin-bound fibronectin (Fn) by inflammatory polymorphonuclear leukocytes (PMNL): A role for leukocyte elastase

Conference · Mon Feb 26 04:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6009781

Daudi, I; Gudewicz, P W; Saba, T M; Cho, E; Lewis, M ^[1]

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1. Albany Medical Coll., NY (United States)

Fragmentation of lung matrix Fn by proteases released from activated leukocytes sequestered in the lung has been implicated in lung vascular injury. The authors determined if Fn bound to a denatured collagen (gelatin) surface was susceptible to degradation by inflammatory PMNLs. Tissue culture wells coated with 1.5% denatured collagen (2 ml/well) prior to the addition of rat peritonal exudate cells, harvested 16 hours after i.p. sterile casein. Inflammatory PMNLs (1 {times} 10{sup 6}) stimulated with zymosan (1 mg) released 3 times more {sup 125}I-Fn into the culture media (DMEM) during a 4 hour incubation as compared to unstimulated PMNLs (2885{plus minus}95 cpm vs 1027{plus minus}82 cpm/100 ul). {sup 125}I-Fn released by stimulated PMNLs was markedly blocked by addition of a leukocyte elastase inhibitor (AAPVCK), moderately blocked by a trypsin inhibitor (TLCK), and not blocked by a thrombin inhibitor (Hirudin). Western blot analysis demonstrated fragmentation of released Fn. Thus, Fn complexed with denatured collagen is susceptible to proteolysis by stimulated inflammatory PMNLs. This may contribute to lung vascular injury with sepsis and intravascular coagulation which elicit sequestration of activated PMNLs in the lung.

• OSTI ID: 6009781
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
• Country of Publication: United States
• Language: English

Related Subjects

550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HYDROLASES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LUNGS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PATHOLOGICAL CHANGES
PEPTIDE HYDROLASES
PROTEINS
PROTEOLYSIS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
SYMPTOMS
TRACER TECHNIQUES