Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Matrix fibronectin disruption and altered endothelial cell adhesion induced by activated leukocytes

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5447472
; ; ;
Sequestration of activated leukocytes (PMN) within the lung may contribute to pulmonary vascular injury following trauma, sepsis, or intravascular coagulation. Monolayers of cultured rat endothelial cells were utilized to evaluate the effect of activated PMNs on endothelial cell attachment and the extracellular fibronectin matrix over a 4 hr incubation interval. Rat endothelial cells were identified by immunofluorescent staining of Factor VIII R:Ag. Endothelial cells were labeled with /sup 51/Cr in order to establish a cell injury assay in which the release of pelletable (cell associated) or non-pelletable activity was measured in the media. PMN activation was verified by chemiluminescence activity. Following phorbol myristate acetate (PMA) the leukocytes aggregated, chemiluminesced, and caused detachment of /sup 51/Cr endothelial cells. Endothelial detachment increased as a function of time with a plateau by 3 hrs. Immunofluorescent analysis of extracellular fibronectin in endothelial cell cultures revealed disruption of the fibrillar matrix fibronectin in association with endothelial cell disadhesion. Matrix fibronectin disruption was not seen with PMNs or PMA alone. Thus, disruption of the fibronectin matrix by released proteases may contribute to endothelial cell detachment.
Research Organization:
Albany Medical College, NY
OSTI ID:
5447472
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

Similar Records

Fragmentation of gelatin-bound fibronectin (Fn) by inflammatory polymorphonuclear leukocytes (PMNL): A role for leukocyte elastase
Conference · Sun Feb 25 23:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States) · OSTI ID:6009781
Fibronectin production by human mammary cells
Journal Article · Wed Dec 31 23:00:00 EST 1980 · J. Natl. Cancer Inst.; (United States) · OSTI ID:5177905
Attenuation of tumor necrosis factor-induced endothelial cell cytotoxicity and neutrophil chemiluminescence
Journal Article · Wed Oct 31 23:00:00 EST 1990 · American Review of Respiratory Disease (New York); (USA) · OSTI ID:6272085

Related Subjects

550901 -- Pathology-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADHESION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CELL CULTURES
CHROMIUM 51
CHROMIUM ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ESTERS
EVEN-ODD NUCLEI
FLUORESCENCE
GLUCOPROTEINS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LUMINESCENCE
LUNGS
MAMMALS
MATERIALS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PHORBOL ESTERS
PROTEINS
RADIOISOTOPES
RATS
RESPIRATORY SYSTEM
RODENTS
SACCHARIDES
TISSUES
TRACER TECHNIQUES
VERTEBRATES