Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Triacylglycerol secretion in rats: validation of a tracer method employing radioactive glycerol

Journal Article · · J. Nutr.; (United States)
OSTI ID:6001475
; ;
A two-compartment model was developed to analyze the temporal changes in plasma triacylglycerol (TG)-specific radioactivity after injection of (2-/sup 3/H)glycerol into rats. The analysis, which yielded fractional rate constants of TG secretion, was tested in rats fed diets either adequate or deficient in essential fatty acids (EFA) and containing either glucose, fructose or sucrose as the dietary carbohydrate. The method of analysis appeared valid, first, because of a close agreement between experimental and computer-fitted TG-specific radioactivity curves, and second, because the fractional rate constants obtained were quite similar to fractional rate constants determined previously by the Triton WR-1339 technique in rats maintained on identical diets. The results show that EFA deficiency increased the fractional rate constant of TG secretion 1.7-, 1.8- and 3.3-fold and the rate of TG secretion 1.8-, 1.6- and 1.4-fold when the dietary carbohydrate was glucose, sucrose and fructose, respectively, in comparison with control rats fed diets supplying these same carbohydrates but adequate in EFA. In the latter groups, the rates of plasma TG secretion were in the range of 0.14-0.17 mg/min per 100 g body weight, and the rate of secretion in the fructose-fed rats was only 20% higher than in the glucose-fed rats.
Research Organization:
Univ. of California, Berkeley
OSTI ID:
6001475
Journal Information:
J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 114:10; ISSN JONUA
Country of Publication:
United States
Language:
English

Similar Records

Fetal polyol metabolism in copper deficiency
Journal Article · Wed Feb 08 23:00:00 EST 1989 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA) · OSTI ID:6832852
Effect of dietary copper and sucrose on catecholamine concentrations in the adrenal medulla
Conference · Sun Mar 10 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States) · OSTI ID:5371818
Effects of dietary carbohydrate on iron metabolism and cytochrome oxidase activity in copper-deficient rats
Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5381840

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
GLYCEROL
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NUTRITIONAL DEFICIENCY
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOACTIVITY
RATS
RODENTS
SECRETION
TESTING
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VALIDATION
VERTEBRATES