Temporal acquisition of enhanced fibrinolytic activity by Syrian hamster embryo cells following treatment with benzo(a)pyrene

Journal Article · Sat Oct 01 00:00:00 EDT 1977 · Cancer Res.; (United States)

OSTI ID:5998537

Barrett, J C ^[1]; Crawford, B D; Grady, D L; Hester, L D; Jones, P A; Benedict, W F; Ts'o, P O.P.

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1. Johns Hopkins Univ., Baltimore, MD

Following treatment of Syrian hamster embryo cells with benzo(a)pyrene, the time required for the expression of enhanced fibrinolytic activity was examined. For this study, the fibrin-agarose overlay method was developed to distinguish the activity of normal and transformed colonies of hamster cells. Colonies possessing enhanced fibrinolytic activity were not observed 8 days after treatment, but they were observed following one passage (2 weeks after treatment). Morphologically transformed colonies, which exhibited no enhanced fibrinolytic activity, were observed 8 days following treatment. In contrast to these two early changes, cells capable of growth in soft agar were observed much later (6 to 8 weeks after treatment). Untreated Syrian hamster embryo cells generally senesced and did not exhibit enhanced fibrinolytic activity. Approximately 1 of 10 untreated cultures escaped senescence and evolved as a continuous cell line; such cultures frequently exhibited enhanced fibrinolytic activity. These results suggest that the acquisition of enhanced fibrinolytic activity, while perhaps not a cause of neoplastic transformation, may reflect a loss of control of the normal function of the cellular genetic apparatus during the process of transformation.

• OSTI ID: 5998537
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 37; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550500 -- Metabolism
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGAR
AGING
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BLOOD COAGULATION FACTORS
CARBOHYDRATES
CELL CULTURES
COLLOIDS
CONDENSED AROMATICS
DISEASES
DISPERSIONS
EMBRYONIC CELLS
FIBRIN
GENETIC CONTROL
HAMSTERS
HYDROCARBONS
LYSIS
MAMMALS
METABOLISM
MORPHOLOGICAL CHANGES
NEOPLASMS
ORGANIC COMPOUNDS
PEST CONTROL
POLLUTION
POLYSACCHARIDES
PROTEINS
RODENTS
SACCHARIDES
SCLEROPROTEINS
STRAIN AGING
TIME DEPENDENCE
TRANSFORMATIONS
VERTEBRATES