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Temporal acquisition of enhanced fibrinolytic activity by Syrian hamster embryo cells following treatment with benzo(a)pyrene

Journal Article · · Cancer Res.; (United States)
OSTI ID:5907806
; ; ; ; ; ;
Following treatment of Syrian hamster embryo cells with benzo(a)pyrene, the time required for the expression of enhanced fibrinolytic activity was examined. For this study, the fibrin-agarose overlay method was developed to distinguish the activity of normal and transformed colonies of hamster cells. Colonies possessing enhanced fibrinolytic activity were not observed 8 days after treatment, but they were observed following one passage (2 weeks after treatment). Morphologically transformed colonies, which exhibited no enhanced fibrinolytic activity, were observed 8 days following treatment. In contrast to these two early changes, cells capable of growth in soft agar were observed much later (6 to 8 weeks after treatment). Untreated Syrian hamster embryo cells generally senesced and did not exhibit enhanced fibrinolytic activity. Approximately 1 of 10 untreated cultures escaped senescence and evolved as a continuous cell line; such cultures frequently exhibited enhanced fibrinolytic activity. These results suggest that the acquisition of enhanced fibrinolytic activity, while perhaps not a cause of neoplastic transformation, may reflect a loss of control of the normal function of the cellular genetic apparatus during the process of transformation.
Research Organization:
Johns Hopkins Univ., Baltimore, MD
OSTI ID:
5907806
Journal Information:
Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 37; ISSN CNREA
Country of Publication:
United States
Language:
English

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Related Subjects

550300 -- Cytology
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BIOLOGY
CELL CULTURES
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
EMBRYOS
FIBRINOLYSIS
GENETICS
HAMSTERS
HYDROCARBONS
MAMMALS
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
PROTEOLYSIS
RODENTS
VERTEBRATES