Trace level analysis of mercury using urease in combination with an ammonia gas sensitive semiconductor structure
Journal Article
·
· Anal. Lett.; (United States)
A method for the determination of mercury(II)ions at trace levels is described. The method is based on the profound inhibitory effect of mercury on the enzyme urease. The activity of the enzyme was determined by the rate of ammonia produced from urea as followed by an ammonia gas sensitive iridium thin metal film-oxide-semiconductor (IrTMOS) structure. Two systems were investigated. For the initial urease activity studies, a simple microcell was used. Also a test plate, containing dry reagent strips with all necessary chemicals was developed, making the analytical procedure very simple to perform. The test volume applied was 2 ..mu..l and the sensitivity to standard of mercury(II) ions is at least 0.005..mu..M (1.0 ng/ml). One sample could be analyzed in less than 8 minutes. Furthermore, the kinetics of sensor response versus enzyme activity is discussed.
- Research Organization:
- Linkoeping Univ. (Sweden)
- OSTI ID:
- 5996484
- Journal Information:
- Anal. Lett.; (United States), Journal Name: Anal. Lett.; (United States) Vol. 21:10; ISSN ANALB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
500200 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
520200 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
AMIDINASES
AMMONIA
CAPACITANCE
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
COMPLEXES
DATA
ELECTRICAL PROPERTIES
ELEMENTS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
EXPERIMENTAL DATA
HYDRIDES
HYDROGEN COMPOUNDS
HYDROLASES
INFORMATION
IRIDIUM COMPLEXES
MATERIALS
MERCURY
METALS
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NON-PEPTIDE C-N HYDROLASES
NUMERICAL DATA
OXIDES
OXYGEN COMPOUNDS
PHYSICAL PROPERTIES
QUANTITATIVE CHEMICAL ANALYSIS
SEMICONDUCTOR MATERIALS
TRACE AMOUNTS
TRANSITION ELEMENT COMPLEXES
UREASE
400102* -- Chemical & Spectral Procedures
500200 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
520200 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
AMIDINASES
AMMONIA
CAPACITANCE
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
COMPLEXES
DATA
ELECTRICAL PROPERTIES
ELEMENTS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
EXPERIMENTAL DATA
HYDRIDES
HYDROGEN COMPOUNDS
HYDROLASES
INFORMATION
IRIDIUM COMPLEXES
MATERIALS
MERCURY
METALS
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NON-PEPTIDE C-N HYDROLASES
NUMERICAL DATA
OXIDES
OXYGEN COMPOUNDS
PHYSICAL PROPERTIES
QUANTITATIVE CHEMICAL ANALYSIS
SEMICONDUCTOR MATERIALS
TRACE AMOUNTS
TRANSITION ELEMENT COMPLEXES
UREASE