X-ray absorption spectroscopic evidence for binding of the competitive inhibitor 2-mercaptoethanol to the nickel sites of Jack bean urease. A new Ni-Ni interaction in the inhibited enzyme
Journal Article
·
· Inorganic Chemistry; (USA)
- Dartmouth College, Hanover, NH (USA)
- Univ. of Georgia, Athens (USA)
The enzyme Jack bean urease has been identified as the first nickel-containing metalloenzyme to catalyze the hydrolysis of urea to carbon dioxide and ammonia. Competitive inhibitors such as 2-mercaptoethanol (2-ME) have been shown to dramatically affect the ground-state electronic properties of the urease Ni(II) ions. Results of preliminary structural investigations using x-ray absorption spectroscopy of the nickel salts of urease in its native and 2-ME bound forms are presented. The binding of 2-ME to Ni(II) through the thiolate sulfur is confirmed by the results of this study. 17 refs., 2 figs., 2 tabs.
- DOE Contract Number:
- AC03-82ER13000
- OSTI ID:
- 6910362
- Journal Information:
- Inorganic Chemistry; (USA), Journal Name: Inorganic Chemistry; (USA) Vol. 29:4; ISSN 0020-1669; ISSN INOCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
10 SYNTHETIC FUELS
100200* -- Synthetic Fuels-- Production-- (1990-)
ABSORPTION SPECTROSCOPY
AMIDES
AMIDINASES
AMMONIA
CARBONIC ACID DERIVATIVES
CATALYSIS
CHEMICAL PREPARATION
CHEMICAL REACTIONS
COMPLEXES
DATA
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ENZYMES
EXPERIMENTAL DATA
HYDRIDES
HYDROGEN COMPOUNDS
HYDROLASES
HYDROLYSIS
INFORMATION
IONIZING RADIATIONS
LYSIS
NICKEL COMPLEXES
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NON-PEPTIDE C-N HYDROLASES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOMETALLIC COMPOUNDS
PLANTS
RADIATIONS
SOLVOLYSIS
SPECTROSCOPY
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRANSITION ELEMENT COMPLEXES
UREA
UREASE
X RADIATION
100200* -- Synthetic Fuels-- Production-- (1990-)
ABSORPTION SPECTROSCOPY
AMIDES
AMIDINASES
AMMONIA
CARBONIC ACID DERIVATIVES
CATALYSIS
CHEMICAL PREPARATION
CHEMICAL REACTIONS
COMPLEXES
DATA
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ENZYMES
EXPERIMENTAL DATA
HYDRIDES
HYDROGEN COMPOUNDS
HYDROLASES
HYDROLYSIS
INFORMATION
IONIZING RADIATIONS
LYSIS
NICKEL COMPLEXES
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NON-PEPTIDE C-N HYDROLASES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOMETALLIC COMPOUNDS
PLANTS
RADIATIONS
SOLVOLYSIS
SPECTROSCOPY
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRANSITION ELEMENT COMPLEXES
UREA
UREASE
X RADIATION