Disposable cuvette test for enzymatic determination of heavy metals
Conference
·
OSTI ID:153224
- Karl-Franzens Univ., Graz (Austria). Institute of Organic Chemistry
The authors report on an optical cuvette test for total heavy metals based on the inhibition of the enzyme urease by metals ions including silver(I), mercury(II), copper(II), nickel(II), cobalt(II), and cadmium(II). The enzymatic action is monitored using an optical ammonia transducer deposited on the wall of a disposable cuvette. This results in a rapid and inexpensive single-shot device for heavy metal sensing. A solution of urease and buffer is placed in the cuvette with the ammonium sensor membrane fixed on one of its walls. Enzymatic action starts after addition of a defined quantity of urea. This is indicated by the increase in the absorption of the ammonia sensor membrane whose color changes from yellow to blue. The slope of the increase in signal is the information for the un-inhibited reaction. After several minutes, the sample (containing the heavy metal) is added to the cuvette. Heavy metal ions inhibit the enzyme (by binding to the sulfhydryl groups) and cause a decrease in the slope. The ratio of slopes of un-inhibited and inhibited reactions is a direct parameter for detection and calculation total heavy metals.
- OSTI ID:
- 153224
- Report Number(s):
- CONF-9506232--; ISBN 0-8194-1862-5
- Country of Publication:
- United States
- Language:
- English
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