Phosphorylation of a 36,000 M/sub r/ cellular protein in cells infected with partial transformation mutants of Rous sarcoma virus

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.2.147· OSTI ID:5996257

Nakamura, K D; Weber, M J

The authors isolated and characterized mutants of Rous sarcoma virus which induce some parameters of transformation but fail to fully induce other parameters. They believe these mutants code for a pp60/sup src/ which phosphorylates some targets well but phosphorylates others poorly. Using these mutants, they examined the phosphorylation of a 36,000 M/sub r/ protein which is phosphorylated on a tyrosine in cells transformed by Rous sarcoma virus, in an attempt to correlate this phosphorylation with the expression of specific transformation parameters. The authors found that phosphorylation of the 36,000 M/sub r/ protein was neither necessary nor sufficient for loss of fibronectin or for loss of density-dependent inhibition of growth. Phosphorylation of the protein was not sufficient for morphological alterations, increased hexose transport, or loss of adhesiveness. For the parameters measured, the best correlation was with increased plasminogen activator. In addition, it is noteworthy that cells infected with the mutant CU2 displayed low levels of phosphorylation of the 36,000 M/sub r/ protein and also were deficient in anchorage- independent growth and tumorigenicity, raising the possibility that the phosphorylation of the 36,000 M/sub r/ protein may be required for malignant growth properties.

• Research Organization: Dept. of Microbiology, Univ. of Illinois, Urbana, IL 61801
• OSTI ID: 5996257
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 2:2; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

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550400* -- Genetics
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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
CARBOXYLIC ACIDS
CELL TRANSFORMATIONS
CHEMICAL REACTIONS
CODONS
CORRELATIONS
DISEASES
DRUGS
ENZYME ACTIVITY
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
HYDROXY ACIDS
INFECTIOUS DISEASES
MICROORGANISMS
MOLECULAR BIOLOGY
MORPHOLOGY
MUTANTS
NEOPLASMS
ONCOGENIC TRANSFORMATIONS
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHOPROTEINS
PHOSPHORYLATION
PLASMINOGEN
PROTEINS
SARCOMAS
TYROSINE
VIRAL DISEASES
VIRUSES