Ozone toxicity effects on normal cultured lung cells and combined effects of ozone and antitumor drugs on normal and tumor cells

Thesis/Dissertation · Sat Jan 01 00:00:00 EST 1983

OSTI ID:5994661

Morgan, D L.W.

A system was developed for investigating the effects of ozone (O/sub 3/) on cultured cells. Numerous factors were evaluated in designing this in vitro system and several factors, including composition of the liquid phase, depth of liquid covering cells and gas-liquid reaction products, were found to contribute significantly to the toxicity of O/sub 3/ for cultured rat lung fibroblasts (F-cells). Cell injury caused by different in vitro O/sub 3/ exposure methods was shown to be mediated by different toxic species. Cell injury, was mediated primarily by H/sub 2/O/sub 2/ with some contribution by O/sub 2//sup -/ and OH - depending upon the exposure method. Serum was found to protect from many of these O/sub 3/ reaction products by mechanisms involving protein-bound and free sulfhydryls. Combined treatment with O/sub 3/ and antitumor drugs was investigated for potential selective toxicity for tumor cells. Of the drugs tested, only vitamin K/sub 3/ (Vit K) produced a synergistic increase in toxicity for Walker rat carcinoma (WRC) cells when administered prior to or during O/sub 3/ exposure.

• OSTI ID: 5994661
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
OZONE
BIOLOGICAL EFFECTS
AIR POLLUTION
CELL CULTURES
COMPARATIVE EVALUATIONS
LUNGS
RATS
RESPONSE MODIFYING FACTORS
TOXICITY
VITAMIN K
ANIMALS
AROMATICS
BODY
COAGULANTS
DRUGS
HEMATOLOGIC AGENTS
HEPARIN ANTAGONISTS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
POLLUTION
QUINONES
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
VITAMINS
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)