Simultaneous measurement of local glucose utilization and blood flow in the rat brain: an autoradiographic method using two tracers labeled with /sup 14/C

Journal Article · Tue Mar 01 04:00:00 EST 1983 · J. Cereb. Blood Flow Metab.; (United States)

DOI:https://doi.org/10.1038/jcbfm.1983.7· OSTI ID:5994277

Furlow, Jr, T W; Martin, R M; Harrison, L E

A simplified technique that uses two radionuclide tracers has been devised to measure local cerebral glucose utilization (ICGU) and local cerebral blood flow (ICBF) in the same rat. The method employs (/sup 14/C)-2-deoxyglucose and (/sup 14/C)iodoantipyrine to produce an autoradiogram before and another after extraction into chloroform of the (/sup 14/C)iodoantipyrine from the brain sections. The chloroform-extracted autoradiogram yields ICGU, and the difference in tissue /sup 14/C concentration between the two autoradiograms permits calculation of ICBF. The double-isotope technique provides values of ICGU and ICBF that are statistically indistinguishable from those derived from conventional single-isotope methods.

• Research Organization: Department of Neurology, University of Alabama in Birmingham
• OSTI ID: 5994277
• Journal Information: J. Cereb. Blood Flow Metab.; (United States), Journal Name: J. Cereb. Blood Flow Metab.; (United States) Vol. 3:1; ISSN JCBMD
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
AUTORADIOGRAPHY
BLOOD FLOW
BODY
BRAIN
CARBOHYDRATES
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
CEREBRUM
DIAGNOSIS
DIAGNOSTIC USES
GLUCOSE
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PHYSIOLOGY
RATS
RODENTS
SACCHARIDES
TRACER TECHNIQUES
USES
VERTEBRATES