Evaluation of a double-tracer autoradiographic technique for the measurement of both local cerebral glucose metabolism and local cerebral blood flow

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · J. Cereb. Blood Flow Metab.; (United States)

DOI:https://doi.org/10.1038/jcbfm.1985.43· OSTI ID:5019939

Jones, S C; Greenberg, J H

A solvent washout technique is evaluated that could be used to remove one /sup 14/C tracer after a combined autoradiographic CMRglu and CBF study using (/sup 14/C)2-deoxyglucose (2DG) and (/sup 14/C)iodoantipyrine (IAP). The technique permits the simultaneous measurement of local CBF and local CMRglu in the same animal without the use of the short-lived tracers of iodine, /sup 123/I and /sup 131/I, for iodoantipyrine labeling. This report shows that brain tissue sections did not retain /sup 14/C from (/sup 14/C)2DG when chloroform was used as the washout solvent. Chloroform washing removes nearly all the /sup 14/C from (/sup 14/C)IAP. For this study, careful attention was given to the control, prewash measurement of /sup 14/C concentration.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• OSTI ID: 5019939
• Journal Information: J. Cereb. Blood Flow Metab.; (United States), Journal Name: J. Cereb. Blood Flow Metab.; (United States) Vol. 2; ISSN JCBMD
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ANTIPYRETICS
ANTIPYRINE
AUTORADIOGRAPHY
AZOLES
BLOOD FLOW
BODY
BRAIN
CARBOHYDRATES
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DRUGS
GLUCOSE
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HEXOSES
IODINE COMPOUNDS
LABELLED COMPOUNDS
METABOLISM
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRAZOLES
PYRAZOLINES
SACCHARIDES