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Gene-locus mutation assays in diploid human lymphoblast lines

Journal Article · · Chem. Mutagens; (United States)
OSTI ID:5992615
; ;
Our primary reasons for using diploid human lymphoblast lines in studies of mutagenesis are that they can be grown in free suspension: they appear to be genetically stable for chromosome number as well as immortal; and they are readily derived from humans of different genetic backgrounds and possibly different sensitivities to mutagenic agents. Of these properties, the most important in assessing the mutagenicity of a large number of suspect chemicals is the lymphoblasts' ability to grow in suspension culture. In this chapter, we will introduce the basics of lymphoblast husbandry and some protocols (tricks) that we have used to facilitate their use in studying genetic change.
Research Organization:
Massachusetts Inst. of Technology, Cambridge
OSTI ID:
5992615
Journal Information:
Chem. Mutagens; (United States), Journal Name: Chem. Mutagens; (United States) Vol. 6:22; ISSN CMMUA
Country of Publication:
United States
Language:
English

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Related Subjects

550401 -- Genetics-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZINES
BENZOPYRENE
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
COLONY FORMATION
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
ENZYMES
GENETIC EFFECTS
GLYCOSYL TRANSFERASES
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY COMPOUNDS
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MUTAGEN SCREENING
MUTAGENESIS
MUTANTS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PENTOSYL TRANSFERASES
PRIMATES
PURINES
PYRIMIDINES
RADIATIONS
REVIEWS
RIBOSIDES
SCREENING
SOMATIC CELLS
SULFONATES
THYMIDINE
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES