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Mechanism of prolactin action on Nb/sub 2/ node lymphoma cells

Thesis/Dissertation ·
OSTI ID:5990410
The post-receptor mechanism of action by which the pituitary hormone prolactin stimulates cell division was investigated in the Nb/sub 2/ rat lymphoma cell. The replication of Nb/sub 2/ rat lymphoma cells is specifically stimulated by prolactin in a dose dependent manner in the concentration range from 50 pg/ml to 1 ng/ml. Prolactin stimulates proteins synthesis in these cells between 0 and 2 hrs; DNA synthesis between 12 and 16 hrs, and cell replication between 16 and 24 hrs. The effect of prolactin on phospholipid metabolism was tested by measuring the rate of incorporation of (/sup 3/H) myo-inositol, (/sup 3/H) choline and (/sup 14/C) acetate over a 14 hr period. Two significant increases in phospholipid metabolism occur from 30-90 min and from 3-8 hrs. Only the increase in phosphoinositide metabolism was shown to be non-transient. The role of the phospholipase enzymes in the prolactin stimulation of mitogenesis was investigated in the Nb/sub 2/ lymphoma cells.
Research Organization:
Wayne State Univ., Detroit, MI (USA)
OSTI ID:
5990410
Country of Publication:
United States
Language:
English