Technetium-99m-human polyclonal IgG radiolabeled via the hydrazino nicotinamide derivative for imaging focal sites of infection in rats

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Journal of Nuclear Medicine; (USA)

OSTI ID:5987730

Abrams, M J; Juweid, M; tenKate, C I; Schwartz, D A; Hauser, M M; Gaul, F E; Fuccello, A J; Rubin, R H; Strauss, H W; Fischman, A J ^[1]

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1. Massachusetts General Hospital, Boston (USA)

The biologic behavior of human polyclonal immunoglobulin (IgG) radiolabeled with technetium-99m ({sup 99m}Tc) by a novel method, via a nicotinyl hydrazine derivative, was evaluated in rats. Technetium-99m- and indium-111-IgG were co-administered to normal rats and biodistribution was determined at 2, 6, and 16 hr. The inflammation imaging properties of the two reagents were compared in rats with deep-thigh infection due to Escherichia coli. Blood clearance of both antibody preparations was well described by a bi-exponential function: ({sup 99m}Tc-IgG: t1/2 = 3.82 +/- 0.89 and 57.52 +/- 1.70 hr. {sup 111}In-IgG: 3.93 +/- 0.117 and 40.71 +/- 1.26 hr). Biodistributions in the solid organs were similar, however, small but statistically significant differences were detected: {sup 99m}Tc-IgG greater than {sup 111}In-IgG in lung, liver, and spleen; {sup 99m}Tc-IgG less than {sup 111}In-IgG in kidney and skeletal muscle (p less than 0.01). At all three imaging times, target-to-background ratio and percent residual activity for the two compounds were remarkably similar. These studies establish that human polyclonal IgG labeled with {sup 99m}Tc via a nicotinyl hydrazine modified intermediate is equivalent to {sup 111}In-IgG for imaging focal sites of infection in experimental animals.

• OSTI ID: 5987730
• Journal Information: Journal of Nuclear Medicine; (USA), Vol. 31:12; ISSN 0161-5505
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BACTERIAL DISEASES
DIAGNOSIS
IMMUNOGLOBULINS
TISSUE DISTRIBUTION
BLOOD-PLASMA CLEARANCE
ESCHERICHIA COLI
HYDRAZINE
IMAGE PROCESSING
INDIUM 111
ISOMERIC NUCLEI
MAN
RATS
TECHNETIUM 99
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CLEARANCE
DAYS LIVING RADIOISOTOPES
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HOURS LIVING RADIOISOTOPES
INDIUM ISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NITROGEN COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROCESSING
PROTEINS
RADIOISOTOPES
RODENTS
TECHNETIUM ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics