Localization of Fc and Fab fragments of nonspecific polyclonal IgG at focal sites of inflammation

Journal Article · Sun Jul 01 04:00:00 EDT 1990 · Journal of Nuclear Medicine; (USA)

OSTI ID:6609555

Fischman, A J; Rubin, R H; White, J A; Locke, E; Wilkinson, R A; Nedelman, M; Callahan, R J; Khaw, B A; Strauss, H W ^[1]

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1. Massachusetts General Hospital, Boston (USA)

Intact IgG, Fc, Fab, and 1/2Fc (reduced and alkylated Fc) were coupled to DTPA, labeled with indium-111 and administered to rats to compare the ability of fragments of IgG to localize at focal sites of inflammation. Two sets of experiments were performed: First, 1, 6, 24, and 48 hr after injection, biodistribution was determined in healthy animals; second, localization at sites of inflammation was determined by scintillation camera imaging of animals with deep-thigh infection due to Escherichia coli. The biodistribution studies demonstrated differences in kidney and liver localization: IgG less than Fc less than Fab less than 1/2Fc (kidney), Fc less than 1/2Fc less than IgG less than Fab (liver). The imaging studies revealed that target-to-background ratio (T/B) and percent residual activity (%RA) for IgG was significantly greater (p less than 0.01) than 1/2 Fc or Fab, and T/B for IgG was greater (p less than 0.01) than Fc. These studies suggest that the Fc portion of IgG is the fragment with the best imaging properties.

• OSTI ID: 6609555
• Journal Information: Journal of Nuclear Medicine; (USA), Journal Name: Journal of Nuclear Medicine; (USA) Vol. 31:7; ISSN 0161-5505; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
BACTERIA
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
CARBOXYLIC ACIDS
CHELATING AGENTS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
DRUGS
DTPA
ELECTRON CAPTURE RADIOISOTOPES
ESCHERICHIA COLI
GLANDS
GLOBULINS
IMMUNOGLOBULINS
INDIUM 111
INDIUM ISOTOPES
INFECTIOUS DISEASES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIVER
MAMMALS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROTEINS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
SCINTISCANNING
SPLEEN
SYMPTOMS
TISSUE DISTRIBUTION
VERTEBRATES