Autoradiographic method for quantitation of radiolabeled proteins in tissues using indium-111

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Journal of Nuclear Medicine; (USA)

OSTI ID:5362124

Morrell, E M; Tompkins, R G; Fischman, A J; Wilkinson, R A; Burke, J F; Rubin, R H; Strauss, H W; Yarmush, M L ^[1]

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1. Massachusetts General Hospital, Boston (USA)

A quantitative autoradiographic method was developed to measure 111In-labeled proteins in extravascular tissues with a spatial resolution sufficient to associate these proteins with tissue morphology. A linear relationship between measured grain density and isotope concentration was demonstrated with uniformly-labeled standard sources of epoxy-embedded gelatin containing (111In)albumin; half-distance of spatial resolution was 0.6 micron. The technique was illustrated by measuring 24-hr accumulation of diethylenetriaminepentaacetic acid-coupled 111In-labeled human polyclonal IgG and human serum albumin (HSA) in a thigh infection model in the rat. Gamma camera images localized the infection and showed target-to-background ratios of 2.5 {plus minus} 0.3 for IgG and 1.4 {plus minus} 0.02 for human serum albumin (mean {plus minus} s.d., n = 3). Using quantitative autoradiography, significantly higher average tissue concentrations were found in the infected thighs at 4 to 4.5% of the initial plasma concentrations as compared to 0.2 to 0.3% of initial plasma concentrations in the noninfected thigh (p less than 0.05); these radiolabeled proteins were not inflammatory cell associated and localized primarily within the edematous interstitial spaces of the infection.

• OSTI ID: 5362124
• Journal Information: Journal of Nuclear Medicine; (USA), Journal Name: Journal of Nuclear Medicine; (USA) Vol. 30:9; ISSN 0161-5505; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOLOGICAL MODELS
CAMERAS
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GAMMA CAMERAS
IMAGE PROCESSING
INDIUM 111
INDIUM ISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROCESSING
PROTEINS
RADIOISOTOPES
RADIOPHARMACEUTICALS
RATS
RESOLUTION
RODENTS
SPATIAL RESOLUTION
VERTEBRATES