Studies on radiolabeled streptavidin-biotin and its use as an indirect immunoimaging system

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:7063539

Driver, M S

The purpose of this research was to study the biotin-streptavidin system coupled to an antigen-antibody system to amplify the sensitivity of tumor detection. Human serum albumin (HSA) was used as an antigen and conjugated to Sepharose 4B beads. The beads-HSA were used as a tumor model. Rabbit-anti human serum albumin antibody (Ab) was used as antibody and derivatized with biotin (AbXB). Streptavidin (STA) radiolabeled with I-125 and In-111 was used to detect the antigenic sites. The three areas of research included chemical studies, in vitro binding studies, and biodistribution studies. The conjugation reactions of biotin to Ab, diethylenetriaminepentaacetic acid (DTPA) to STA, and the radiolabeling of STA conjugates with In-111 were investigated. The in vitro binding assay was performed in two steps. The AbXB was bound first to beads-HSA in excess. The unbound AbXB was removed and a molar excess of beads-HSA/AbXB was then incubated with I-125 or In-111 labeled STA. For the biodistribution studies, AbXB was injected into a tail vein of rats with beads-HSA localized in lungs. The In-111 or I-125 labeled STA was injected 24 and 48 hours after the administration of the AbXB. These biodistribution studies indicated no amplification of the target to normal organ ratios as compared to In-111 labeled antibody.

• Research Organization: George Washington Univ., Washington, DC (USA)
• OSTI ID: 7063539
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DISTRIBUTION
DRUGS
DTPA
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
IMAGE PROCESSING
IMMUNOASSAY
IMMUNOLOGY
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MAN
METALS
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROCESSING
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RADIOPHARMACEUTICALS
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
TIN
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES