Effect of DTPA conjugation on the antigen binding activity and biodistribution of monoclonal antibodies against. cap alpha. -fetoprotein

Journal Article · Mon Jul 01 04:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:5410825

Sakahara, H; Endo, K; Nakashima, T; Koizumi, M; Ohta, H; Torizuka, K; Furukawa, T; Ohmomo, Y; Yokoyama, A; Okada, K

Indium-111 (/sup 111/In) labeled monoclonal antibodies (Ab) prepared with a bifunctional chelating agent, diethylenetriaminepentaacetic acid (DTPA), have been used for the radioimmunoimaging of cancer. In the present experiment, using monoclonal Ab against human ..cap alpha..-fetoprotein (AFP) as a model, the authors have studied the effect of DTPA conjugation on the antigen binding activity and the biodistribution in nude mice transplanted with AFP-producing human testicular tumor. In Ab heavily conjugated with DTPA, the Scatchard plot analysis demonstrated that the maximum binding capacity rather than the affinity constant was affected. Under selected conditions, /sup 111/In-labeled Ab were made available with almost full retention of the antigen binding activity and scintigrams of nude mice clearly delineated the site of the tumor. However, the number of DTPA molecules incorporated per Ab molecule markedly influenced the in vivo biodistribution as well as the in vitro antigen binding activity.

• Research Organization: Kyoto Univ., Japan
• OSTI ID: 5410825
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 26:7; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHELATING AGENTS
CHLORIDES
CHLORINE COMPOUNDS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DISTRIBUTION
DRUGS
DTPA
ELECTRON CAPTURE RADIOISOTOPES
HALIDES
HALOGEN COMPOUNDS
INDIUM 111
INDIUM CHLORIDES
INDIUM COMPOUNDS
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RODENTS
SCINTISCANNING
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES