Immunoreactivity and biodistribution of indium-111-labeled monoclonal antibody to a human high molecular weight-melanoma associated antigen

Journal Article · Wed May 01 04:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:5410415

Fawwaz, R A; Wang, T S.T.; Estabrook, A; Rosen, J M; Hardy, M A; Alderson, P O; Srivastava, S C; Richards, P; Ferrone, S

The anti-human, high molecular weight-melanoma associated antigen (HMW-MAA)MoAb 225.28S was chelated with /sup 111/In and then tested for its in vitro reactivity with cultured human melanoma cells and for its biodistribution in human melanoma bearing nude mice. In vitro studies showed that the radiolabeled antibody reacted specifically with cultured melanoma cells. However, binding of DTPA to the monoclonal antibody reduced its titer with cultured melanoma cells from 1:1024 to 1:512. Injection of the radiolabeled monoclonal antibody into nude mice resulted in the accumulation of significantly higher radioactivity in melanoma tissue than in nude mice injected with either (/sup 111/In)chloride or /sup 111/In-labeled antibody to human acid phosphatase. The localization of antibody in liver and kidney was also high, although lower than that achieved in tumor. These results indicate that /sup 111/In-labeled monoclonal antibodies to human tumor associated antigens may be useful for localizing malignant lesions. However, there is a need to improve labeling and/or purification of antibody in order to decrease renal and hepatic activity.

• Research Organization: Columbia Univ., New York
• OSTI ID: 5410415
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 26:5; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHELATES
CHELATING AGENTS
CHEMICAL PROPERTIES
COMPLEXES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
DISTRIBUTION
DRUGS
DTPA
ELECTRON CAPTURE RADIOISOTOPES
IN VITRO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MELANOMAS
MICE
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOISOTOPES
RADIOPHARMACEUTICALS
RADIOPROTECTIVE SUBSTANCES
RODENTS
TISSUE DISTRIBUTION
VERTEBRATES