Measurement of regional cerebral metabolic rate for glucose in the human subject with (F-18)-2-deoxy-2-fluoro-d-glucose and emission computed tomography: validation of the method

Conference · Sat Jan 01 04:00:00 EST 1977

OSTI ID:5987231

Phelps, M E; Huang, S C; Hoffman, E J; Selin, C; Kuhl, D E

Tracer techniques and models of in vitro quantitative autoradiography and tissue counting for the measure of regional metabolic rates (rMR) are combined with emission computed tomography (ECT). This approach, Physiologic Tomography (PT), provides atraumatic and analytical measurements of rMR. PT is exemplified with the regional measurement of the cerebral metabolic rate for glucose (CMRGlu) in man with (/sup 18/F)-2-deoxy-2-fluoro-D-glucose (FDG) and positron ECT. Our model incorporates a k/sub 4/* mediated hydrolysis of FDG-6-PO/sub 4/ to FDG which then competes with phosphorylation (k/sub 3/*) of FDG back to FDG-6-PO/sub 4/ and reverse transport (k/sub 2/*) back to blood. Although small, k/sub 4/* is found to be significant. The ECAT positron tomograph was used to measure the rate constants (k/sub 1/*..-->..k/sub 4/*), lumped constant (LC), stability, and reproducibility of the model in man. Since these parameters have not been measured for FDG in any species, comparisons are made to values for DG in rat and monkey. Compartmental concentrations of FDG and FDG-6-PO/sub 4/ were determined and show that cerebral FDG-6-PO/sub 4/ steadily accumulates for about 100 mins, plateaus and then slowly decreases due to hydrolysis. Cerebral blood FDG concentration was determined to be a minor contribution to tissue activity after 10 min. Regional CMRGlu measurements are reproducible to +- 5.5% over 5 hrs. PT allows the in vivo study ofregional biochemistry and physiology in normal and pathophysiologic states in man with a unique and fundamental capability.

• Research Organization: California Univ., Los Angeles (USA). Lab. of Nuclear Medicine and Radiation Biology
• DOE Contract Number: EY-76-C-03-0012
• OSTI ID: 5987231
• Report Number(s): UCLA-12-1162; CONF-7706182-1
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARBOHYDRATES
CAT SCANNING
CENTRAL NERVOUS SYSTEM
CEREBRUM
COMPARATIVE EVALUATIONS
DIAGNOSTIC TECHNIQUES
DRUGS
ECAT SCANNING
FLUORINE 18
FLUORINE ISOTOPES
GLUCOSE
HEXOSES
HOURS LIVING RADIOISOTOPES
INJECTION
INTAKE
INTRAVENOUS INJECTION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MAN
MATHEMATICAL MODELS
MEASURING METHODS
METABOLISM
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
PHOTON EMISSION SCANNING
PHOTON TRANSMISSION SCANNING
PRIMATES
RADIOACTIVE MATERIALS
RADIOISOTOPES
RADIOPHARMACEUTICALS
REACTION KINETICS
SACCHARIDES
TOMOGRAPHY
TRACER TECHNIQUES
VERTEBRATES